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Sample GSM703896 Query DataSets for GSM703896
Status Public on Jan 23, 2012
Title CD8+ T cells rep3 miRNA (Roche)
Sample type RNA
 
Source name CD8+ T cells from healthy donor pool 12
Organism Homo sapiens
Characteristics cell type: CD8+ T cells
Extracted molecule total RNA
Extraction protocol Total RNA, including small non-coding RNAs, was extracted using the mirVana RNA isolation kit (Ambion, Inc, Austin, TX, USA) according to manufacturer’s protocol. RNA was quantified using a ND-1000 spectrophotometer (Nanodrop Technology®, Cambridge, UK) and RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent technologies, Santa Clara, CA, USA). The 260/280 ratio were between 1.7 and 2.1 and the RINs (RNA Integrity Numbers) greater than 6 for all samples.
Label Biotin
Label protocol Total RNA (300ng) was biotin labeled using the FlashTag™ HSR RNA labeling kit for Affymetrix miRNA arrays (Genisphere LLC., Hatfield, PA, USA) according to manufacturer’s instructions
 
Hybridization protocol Labeled RNA was hybridized to Affymetrix GeneChip miRNA microarrays according to manufacturer’s instructions. Wash and stain protocol FS450_0003.
Scan protocol GeneChips were scanned using the GC 30007G scanner.
Description CD14+ monocytes, CD8+ T cells, CD56+ NK cells and CD19 B cells were isolated using the Human CD14 positive Selection Kit, Human CD8+ T cells Enrichment Kit, EasySep® Human CD56+ NK cells Enrichment Kit, EasySep® Human CD19+ B cells Enrichment Kit (all from Stem Cell Technologies) respectively according to manufacturer’s instructions.
Data processing Data was normalized in R/Bioconductor using the robust multi-array average (RMA) expression measure.
 
Submission date Apr 08, 2011
Last update date Jan 24, 2012
Contact name Donavan Cheng
E-mail(s) donavan.cheng@gmail.com
Organization name Hoffmann-LaRoche
Department Translational Research Sciences
Street address 340 Kingsland Ave
City Nutley
State/province NJ
ZIP/Postal code 07110
Country USA
 
Platform ID GPL8786
Series (2)
GSE28487 miRNA expression profiling of human immune cell subsets (Roche)
GSE28492 miRNA and mRNA expression profiling of human immune cell subsets

Data table header descriptions
ID_REF
VALUE log2 RMA signal intensity

Data table
ID_REF VALUE
14q-0_st 0.58139
14qI-1_st 1.83635
14qI-2_st 0.893048
14qI-3_x_st 0.850827
14qI-4_st 1.37256
14qI-4_x_st 1.84361
14qI-5_st 0.873862
14qI-6_st 0.818109
14qI-6_x_st 1.05788
14qI-7_st 1.05384
14qI-8_st 1.31067
14qI-8_x_st 1.3388
14qI-9_x_st 1.3277
14qII-10_st 0.955978
14qII-11_st 0.948808
14qII-11_x_st 1.51141
14qII-12_st 0.770445
14qII-12_x_st 1.49762
14qII-13_st 0.809562
14qII-14_st 0.843129

Total number of rows: 7815

Table truncated, full table size 183 Kbytes.




Supplementary file Size Download File type/resource
GSM703896_miRNA_CD8_EXP12.CEL.gz 157.0 Kb (ftp)(http) CEL
Processed data included within Sample table

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