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Sample GSM704660 Query DataSets for GSM704660
Status Public on Jan 23, 2012
Title NK cells rep1 miRNA (HUG)
Sample type RNA
 
Source name NK cells from healthy donors
Organism Homo sapiens
Characteristics cell type: NK cells
Extracted molecule total RNA
Extraction protocol Total RNA, including small non-coding RNAs, was extracted using the mirVana RNA isolation kit (Ambion, Inc, Austin, TX, USA) according to manufacturer’s protocol. RNA was quantified using a ND-1000 spectrophotometer (Nanodrop Technology®, Cambridge, UK) and RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent technologies, Santa Clara, CA, USA). The 260/280 ratio were between 1.7 and 2.1 and the RINs (RNA Integrity Numbers) greater than 6 for all samples.
Label Biotin
Label protocol Total RNA (300ng) was biotin labeled using the FlashTag™ HSR RNA labeling kit for Affymetrix miRNA arrays (Genisphere LLC., Hatfield, PA, USA) according to manufacturer’s instructions
 
Hybridization protocol Labeled RNA was hybridized to Affymetrix GeneChip miRNA microarrays according to manufacturer’s instructions. Wash and stain protocol FS450_0003.
Scan protocol GeneChips were scanned using the GC 30007G scanner.
Description Isolated at University Hospital of Geneva
Data processing Data was normalized in R/Bioconductor using the robust multi-array average (RMA) expression measure.
 
Submission date Apr 08, 2011
Last update date Jan 24, 2012
Contact name Donavan Cheng
E-mail(s) donavan.cheng@gmail.com
Organization name Hoffmann-LaRoche
Department Translational Research Sciences
Street address 340 Kingsland Ave
City Nutley
State/province NJ
ZIP/Postal code 07110
Country USA
 
Platform ID GPL8786
Series (2)
GSE28489 miRNA expression profiling of human immune cell subsets (HUG)
GSE28492 miRNA and mRNA expression profiling of human immune cell subsets

Data table header descriptions
ID_REF
VALUE log2 RMA signal intensity

Data table
ID_REF VALUE
14q-0_st 1.4907
14qI-1_st 2.98074
14qI-2_st 1.25395
14qI-3_x_st 1.60455
14qI-4_st 1.59288
14qI-4_x_st 1.37258
14qI-5_st 0.873342
14qI-6_st 1.40783
14qI-6_x_st 1.41622
14qI-7_st 1.40651
14qI-8_st 1.28914
14qI-8_x_st 1.23638
14qI-9_x_st 1.00054
14qII-10_st 1.15619
14qII-11_st 1.25481
14qII-11_x_st 1.25395
14qII-12_st 1.4388
14qII-12_x_st 1.47208
14qII-13_st 1.27453
14qII-14_st 1.24152

Total number of rows: 7815

Table truncated, full table size 182 Kbytes.




Supplementary file Size Download File type/resource
GSM704660_Cell.NK_1_30.04.2010_miRNA-1_0_2Xgain_.CEL.gz 153.5 Kb (ftp)(http) CEL
Processed data included within Sample table

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