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Sample GSM704663 Query DataSets for GSM704663
Status Public on Jan 23, 2012
Title NK cells rep3 miRNA (HUG)
Sample type RNA
 
Source name NK cells from healthy donors
Organism Homo sapiens
Characteristics cell type: NK cells
Extracted molecule total RNA
Extraction protocol Total RNA, including small non-coding RNAs, was extracted using the mirVana RNA isolation kit (Ambion, Inc, Austin, TX, USA) according to manufacturer’s protocol. RNA was quantified using a ND-1000 spectrophotometer (Nanodrop Technology®, Cambridge, UK) and RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent technologies, Santa Clara, CA, USA). The 260/280 ratio were between 1.7 and 2.1 and the RINs (RNA Integrity Numbers) greater than 6 for all samples.
Label Biotin
Label protocol Total RNA (300ng) was biotin labeled using the FlashTag™ HSR RNA labeling kit for Affymetrix miRNA arrays (Genisphere LLC., Hatfield, PA, USA) according to manufacturer’s instructions
 
Hybridization protocol Labeled RNA was hybridized to Affymetrix GeneChip miRNA microarrays according to manufacturer’s instructions. Wash and stain protocol FS450_0003.
Scan protocol GeneChips were scanned using the GC 30007G scanner.
Description Isolated at University Hospital of Geneva
Data processing Data was normalized in R/Bioconductor using the robust multi-array average (RMA) expression measure.
 
Submission date Apr 08, 2011
Last update date Jan 24, 2012
Contact name Donavan Cheng
E-mail(s) donavan.cheng@gmail.com
Organization name Hoffmann-LaRoche
Department Translational Research Sciences
Street address 340 Kingsland Ave
City Nutley
State/province NJ
ZIP/Postal code 07110
Country USA
 
Platform ID GPL8786
Series (2)
GSE28489 miRNA expression profiling of human immune cell subsets (HUG)
GSE28492 miRNA and mRNA expression profiling of human immune cell subsets

Data table header descriptions
ID_REF
VALUE log2 RMA signal intensity

Data table
ID_REF VALUE
14q-0_st 0.965331
14qI-1_st 2.94591
14qI-2_st 1.1034
14qI-3_x_st 1.60573
14qI-4_st 1.90035
14qI-4_x_st 1.34859
14qI-5_st 1.1034
14qI-6_st 1.12198
14qI-6_x_st 1.18685
14qI-7_st 1.07116
14qI-8_st 0.884246
14qI-8_x_st 1.15627
14qI-9_x_st 1.20296
14qII-10_st 1.14917
14qII-11_st 1.05349
14qII-11_x_st 1.15006
14qII-12_st 1.36629
14qII-12_x_st 1.21055
14qII-13_st 1.26072
14qII-14_st 1.3209

Total number of rows: 7815

Table truncated, full table size 182 Kbytes.




Supplementary file Size Download File type/resource
GSM704663_cell.NK_3_20.07.2010_miRNA-1_0_2Xgain_.CEL.gz 158.5 Kb (ftp)(http) CEL
Processed data included within Sample table

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