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Sample GSM704679 Query DataSets for GSM704679
Status Public on Jan 23, 2012
Title Eosinophils rep2 miRNA (HUG)
Sample type RNA
 
Source name Eosinophils from healthy donors
Organism Homo sapiens
Characteristics cell type: Eosinophils
Extracted molecule total RNA
Extraction protocol Total RNA, including small non-coding RNAs, was extracted using the mirVana RNA isolation kit (Ambion, Inc, Austin, TX, USA) according to manufacturer’s protocol. RNA was quantified using a ND-1000 spectrophotometer (Nanodrop Technology®, Cambridge, UK) and RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent technologies, Santa Clara, CA, USA). The 260/280 ratio were between 1.7 and 2.1 and the RINs (RNA Integrity Numbers) greater than 6 for all samples.
Label Biotin
Label protocol Total RNA (300ng) was biotin labeled using the FlashTag™ HSR RNA labeling kit for Affymetrix miRNA arrays (Genisphere LLC., Hatfield, PA, USA) according to manufacturer’s instructions
 
Hybridization protocol Labeled RNA was hybridized to Affymetrix GeneChip miRNA microarrays according to manufacturer’s instructions. Wash and stain protocol FS450_0003.
Scan protocol GeneChips were scanned using the GC 30007G scanner.
Description Isolated at University Hospital of Geneva
Data processing Data was normalized in R/Bioconductor using the robust multi-array average (RMA) expression measure.
 
Submission date Apr 08, 2011
Last update date Jan 24, 2012
Contact name Donavan Cheng
E-mail(s) donavan.cheng@gmail.com
Organization name Hoffmann-LaRoche
Department Translational Research Sciences
Street address 340 Kingsland Ave
City Nutley
State/province NJ
ZIP/Postal code 07110
Country USA
 
Platform ID GPL8786
Series (2)
GSE28489 miRNA expression profiling of human immune cell subsets (HUG)
GSE28492 miRNA and mRNA expression profiling of human immune cell subsets

Data table header descriptions
ID_REF
VALUE log2 RMA signal intensity

Data table
ID_REF VALUE
14q-0_st 1.35866
14qI-1_st 3.31487
14qI-2_st 1.16286
14qI-3_x_st 1.37491
14qI-4_st 1.53853
14qI-4_x_st 1.39634
14qI-5_st 1.25375
14qI-6_st 1.03487
14qI-6_x_st 1.75632
14qI-7_st 1.75929
14qI-8_st 1.16753
14qI-8_x_st 1.65663
14qI-9_x_st 1.27941
14qII-10_st 1.29969
14qII-11_st 1.24817
14qII-11_x_st 1.13435
14qII-12_st 1.07305
14qII-12_x_st 0.910857
14qII-13_st 1.14262
14qII-14_st 1.2283

Total number of rows: 7815

Table truncated, full table size 182 Kbytes.




Supplementary file Size Download File type/resource
GSM704679_miRNA_EOSINO_22102010_miRNA-1_0_2Xgain_.CEL.gz 153.6 Kb (ftp)(http) CEL
Processed data included within Sample table

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