|
Status |
Public on Apr 13, 2011 |
Title |
IL-exp6_hFc_1.4-2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
IL-exp6_hFc_1.4-2 sample
|
Organism |
Mus musculus |
Characteristics |
strain: C57Bl6 tissue: retina treatment: hFc
|
Treatment protocol |
8 day old pups were injected intravitreally (ITV) with 1 microgram VEGFA, 4 micrograms Dll4-Fc, or 4 micrograms hFc alone
|
Growth protocol |
C57Bl6 mice were housed in normal conditions
|
Extracted molecule |
total RNA |
Extraction protocol |
Retinas were extracted from mouse pups 24 hrs after treatment. RNA was purified using Rneasy Mini Kit (Qiagen).
|
Label |
Cy3
|
Label protocol |
Cyanine labeled cRNA was produced from 2 microgram of total RNA using low RNA Input Linear Amplication Kit (Agilent).
|
|
|
Channel 2 |
Source name |
IL-exp6 common reference
|
Organism |
Mus musculus |
Characteristics |
strain: C57Bl6 tissue: retina description: pooled of equal amount of RNA from this experiment
|
Treatment protocol |
8 day old pups were injected intravitreally (ITV) with 1 microgram VEGFA, 4 micrograms Dll4-Fc, or 4 micrograms hFc alone
|
Growth protocol |
C57Bl6 mice were housed in normal conditions
|
Extracted molecule |
total RNA |
Extraction protocol |
Retinas were extracted from mouse pups 24 hrs after treatment. RNA was purified using Rneasy Mini Kit (Qiagen).
|
Label |
Cy5
|
Label protocol |
Cyanine labeled cRNA was produced from 2 microgram of total RNA using low RNA Input Linear Amplication Kit (Agilent).
|
|
|
|
Hybridization protocol |
Each sample labeled in Cyanine 3 and common reference labeled in Cyanine 5 were hybridized to a custom Agilent mouse whole genome gene expression array.
|
Scan protocol |
Scanned on an Agilent Technologies Scanner G2505B scanner.
|
Data processing |
Agilent Feature Extraction Software (v9.5.1.1) was used for background subtraction and LOWESS normalization.
|
|
|
Submission date |
Apr 11, 2011 |
Last update date |
Apr 13, 2011 |
Contact name |
Ivan B Lobov |
E-mail(s) |
Ivan.Lobov@regeneron.com
|
Organization name |
Regeneron Pharmaceuticals
|
Department |
Regeneron Pharmaceuticals
|
Street address |
777 Old Saw Mill River Road,
|
City |
Tarrytown |
State/province |
NY |
ZIP/Postal code |
10591 |
Country |
USA |
|
|
Platform ID |
GPL13397 |
Series (1) |
GSE28516 |
Expression analysis of mouse retinas after treatment with VEGF-A or Dll4-Fc. |
|