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Sample GSM706360 Query DataSets for GSM706360
Status Public on Apr 13, 2011
Title IL-exp6_Dll4-Fc_2.3-2
Sample type RNA
 
Channel 1
Source name IL-exp6_Dll4-Fc_2.3-2 sample
Organism Mus musculus
Characteristics strain: C57Bl6
tissue: retina
treatment: Dll4-Fc
Treatment protocol 8 day old pups were injected intravitreally (ITV) with 1 microgram VEGFA, 4 micrograms Dll4-Fc, or 4 micrograms hFc alone
Growth protocol C57Bl6 mice were housed in normal conditions
Extracted molecule total RNA
Extraction protocol Retinas were extracted from mouse pups 24 hrs after treatment. RNA was purified using Rneasy Mini Kit (Qiagen).
Label Cy3
Label protocol Cyanine labeled cRNA was produced from 2 microgram of total RNA using low RNA Input Linear Amplication Kit (Agilent).
 
Channel 2
Source name IL-exp6 common reference
Organism Mus musculus
Characteristics strain: C57Bl6
tissue: retina
description: pooled of equal amount of RNA from this experiment
Treatment protocol 8 day old pups were injected intravitreally (ITV) with 1 microgram VEGFA, 4 micrograms Dll4-Fc, or 4 micrograms hFc alone
Growth protocol C57Bl6 mice were housed in normal conditions
Extracted molecule total RNA
Extraction protocol Retinas were extracted from mouse pups 24 hrs after treatment. RNA was purified using Rneasy Mini Kit (Qiagen).
Label Cy5
Label protocol Cyanine labeled cRNA was produced from 2 microgram of total RNA using low RNA Input Linear Amplication Kit (Agilent).
 
 
Hybridization protocol Each sample labeled in Cyanine 3 and common reference labeled in Cyanine 5 were hybridized to a custom Agilent mouse whole genome gene expression array.
Scan protocol Scanned on an Agilent Technologies Scanner G2505B scanner.
Data processing Agilent Feature Extraction Software (v9.5.1.1) was used for background subtraction and LOWESS normalization.
 
Submission date Apr 11, 2011
Last update date Apr 13, 2011
Contact name Ivan B Lobov
E-mail(s) Ivan.Lobov@regeneron.com
Organization name Regeneron Pharmaceuticals
Department Regeneron Pharmaceuticals
Street address 777 Old Saw Mill River Road,
City Tarrytown
State/province NY
ZIP/Postal code 10591
Country USA
 
Platform ID GPL13397
Series (1)
GSE28516 Expression analysis of mouse retinas after treatment with VEGF-A or Dll4-Fc.

Data table header descriptions
ID_REF
VALUE lowess normalized natural logarithm of test/reference ratio

Data table
ID_REF VALUE
A_51_P100034 0.079141445
A_51_P100052 -0.11271812
A_51_P100063 0.02435072
A_51_P100065 -0.037128948
A_51_P100084 -0.017071236
A_51_P100099 -0.0788936
A_51_P100101 0.036884658
A_51_P100123
A_51_P100155 0.03637475
A_51_P100174 0.053535294
A_51_P100180 -0.059026245
A_51_P100208 0.15536737
A_51_P100227 -9.30E-04
A_51_P100238
A_51_P100246 0.10420837
A_51_P100289 0.003604346
A_51_P100296 0.012238291
A_51_P100298 0.002030218
A_51_P100309
A_51_P100312 0.1535289

Total number of rows: 43225

Table truncated, full table size 918 Kbytes.




Supplementary file Size Download File type/resource
GSM706360_R_251534710316_S01_R244k_1_2.txt.gz 15.7 Mb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table

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