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Sample GSM706361 Query DataSets for GSM706361
Status Public on Apr 13, 2011
Title IL-exp6_Dll4-Fc_2.4-2
Sample type RNA
 
Channel 1
Source name IL-exp6_Dll4-Fc_2.4-2 sample
Organism Mus musculus
Characteristics strain: C57Bl6
tissue: retina
treatment: Dll4-Fc
Treatment protocol 8 day old pups were injected intravitreally (ITV) with 1 microgram VEGFA, 4 micrograms Dll4-Fc, or 4 micrograms hFc alone
Growth protocol C57Bl6 mice were housed in normal conditions
Extracted molecule total RNA
Extraction protocol Retinas were extracted from mouse pups 24 hrs after treatment. RNA was purified using Rneasy Mini Kit (Qiagen).
Label Cy3
Label protocol Cyanine labeled cRNA was produced from 2 microgram of total RNA using low RNA Input Linear Amplication Kit (Agilent).
 
Channel 2
Source name IL-exp6 common reference
Organism Mus musculus
Characteristics strain: C57Bl6
tissue: retina
description: pooled of equal amount of RNA from this experiment
Treatment protocol 8 day old pups were injected intravitreally (ITV) with 1 microgram VEGFA, 4 micrograms Dll4-Fc, or 4 micrograms hFc alone
Growth protocol C57Bl6 mice were housed in normal conditions
Extracted molecule total RNA
Extraction protocol Retinas were extracted from mouse pups 24 hrs after treatment. RNA was purified using Rneasy Mini Kit (Qiagen).
Label Cy5
Label protocol Cyanine labeled cRNA was produced from 2 microgram of total RNA using low RNA Input Linear Amplication Kit (Agilent).
 
 
Hybridization protocol Each sample labeled in Cyanine 3 and common reference labeled in Cyanine 5 were hybridized to a custom Agilent mouse whole genome gene expression array.
Scan protocol Scanned on an Agilent Technologies Scanner G2505B scanner.
Data processing Agilent Feature Extraction Software (v9.5.1.1) was used for background subtraction and LOWESS normalization.
 
Submission date Apr 11, 2011
Last update date Apr 13, 2011
Contact name Ivan B Lobov
E-mail(s) Ivan.Lobov@regeneron.com
Organization name Regeneron Pharmaceuticals
Department Regeneron Pharmaceuticals
Street address 777 Old Saw Mill River Road,
City Tarrytown
State/province NY
ZIP/Postal code 10591
Country USA
 
Platform ID GPL13397
Series (1)
GSE28516 Expression analysis of mouse retinas after treatment with VEGF-A or Dll4-Fc.

Data table header descriptions
ID_REF
VALUE lowess normalized natural logarithm of test/reference ratio

Data table
ID_REF VALUE
A_51_P100034 0.040801242
A_51_P100052 -0.11556676
A_51_P100063 -0.018334368
A_51_P100065 0.29080585
A_51_P100084 -0.26940736
A_51_P100099 0.13822885
A_51_P100101 0.058170106
A_51_P100123
A_51_P100155 0.13899496
A_51_P100174 -0.037180416
A_51_P100180 0.0428406
A_51_P100208 0.07047475
A_51_P100227 -0.14486343
A_51_P100238
A_51_P100246 0.034919906
A_51_P100289 -0.01169701
A_51_P100296 -0.19336718
A_51_P100298 -0.291595
A_51_P100309
A_51_P100312 -0.060202044

Total number of rows: 43225

Table truncated, full table size 917 Kbytes.




Supplementary file Size Download File type/resource
GSM706361_R_251534710316_S01_R244k_1_1.txt.gz 15.8 Mb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table

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