|
| Status |
Public on Apr 13, 2011 |
| Title |
IL-exp6_VEGF A_4.2-2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
IL-exp6_VEGF A_4.2-2 sample
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl6
tissue: retina
treatment: VEGFA
|
| Treatment protocol |
8 day old pups were injected intravitreally (ITV) with 1 microgram VEGFA, 4 micrograms Dll4-Fc, or 4 micrograms hFc alone
|
| Growth protocol |
C57Bl6 mice were housed in normal conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Retinas were extracted from mouse pups 24 hrs after treatment. RNA was purified using Rneasy Mini Kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
Cyanine labeled cRNA was produced from 2 microgram of total RNA using low RNA Input Linear Amplication Kit (Agilent).
|
| |
|
| Channel 2 |
| Source name |
IL-exp6 common reference
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl6
tissue: retina
description: pooled of equal amount of RNA from this experiment
|
| Treatment protocol |
8 day old pups were injected intravitreally (ITV) with 1 microgram VEGFA, 4 micrograms Dll4-Fc, or 4 micrograms hFc alone
|
| Growth protocol |
C57Bl6 mice were housed in normal conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Retinas were extracted from mouse pups 24 hrs after treatment. RNA was purified using Rneasy Mini Kit (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
Cyanine labeled cRNA was produced from 2 microgram of total RNA using low RNA Input Linear Amplication Kit (Agilent).
|
| |
|
| |
| Hybridization protocol |
Each sample labeled in Cyanine 3 and common reference labeled in Cyanine 5 were hybridized to a custom Agilent mouse whole genome gene expression array.
|
| Scan protocol |
Scanned on an Agilent Technologies Scanner G2505B scanner.
|
| Data processing |
Agilent Feature Extraction Software (v9.5.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Apr 11, 2011 |
| Last update date |
Apr 13, 2011 |
| Contact name |
Ivan B Lobov |
| E-mail(s) |
Ivan.Lobov@regeneron.com
|
| Organization name |
Regeneron Pharmaceuticals
|
| Department |
Regeneron Pharmaceuticals
|
| Street address |
777 Old Saw Mill River Road,
|
| City |
Tarrytown |
| State/province |
NY |
| ZIP/Postal code |
10591 |
| Country |
USA |
| |
|
| Platform ID |
GPL13397 |
| Series (1) |
| GSE28516 |
Expression analysis of mouse retinas after treatment with VEGF-A or Dll4-Fc. |
|
