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Sample GSM706368 Query DataSets for GSM706368
Status Public on Apr 13, 2011
Title IL-exp6_VEGF Trap_3.2-1
Sample type RNA
 
Channel 1
Source name IL-exp6_VEGF Trap_3.2-1 sample
Organism Mus musculus
Characteristics strain: C57Bl6
tissue: retina
treatment: VEGF Trap
Treatment protocol 8 day old pups were injected intravitreally (ITV) with 1 microgram VEGFA, 4 micrograms Dll4-Fc, or 4 micrograms hFc alone
Growth protocol C57Bl6 mice were housed in normal conditions
Extracted molecule total RNA
Extraction protocol Retinas were extracted from mouse pups 24 hrs after treatment. RNA was purified using Rneasy Mini Kit (Qiagen).
Label Cy3
Label protocol Cyanine labeled cRNA was produced from 2 microgram of total RNA using low RNA Input Linear Amplication Kit (Agilent).
 
Channel 2
Source name IL-exp6 common reference
Organism Mus musculus
Characteristics strain: C57Bl6
tissue: retina
description: pooled of equal amount of RNA from this experiment
Treatment protocol 8 day old pups were injected intravitreally (ITV) with 1 microgram VEGFA, 4 micrograms Dll4-Fc, or 4 micrograms hFc alone
Growth protocol C57Bl6 mice were housed in normal conditions
Extracted molecule total RNA
Extraction protocol Retinas were extracted from mouse pups 24 hrs after treatment. RNA was purified using Rneasy Mini Kit (Qiagen).
Label Cy5
Label protocol Cyanine labeled cRNA was produced from 2 microgram of total RNA using low RNA Input Linear Amplication Kit (Agilent).
 
 
Hybridization protocol Each sample labeled in Cyanine 3 and common reference labeled in Cyanine 5 were hybridized to a custom Agilent mouse whole genome gene expression array.
Scan protocol Scanned on an Agilent Technologies Scanner G2505B scanner.
Data processing Agilent Feature Extraction Software (v9.5.1.1) was used for background subtraction and LOWESS normalization.
 
Submission date Apr 11, 2011
Last update date Apr 13, 2011
Contact name Ivan B Lobov
E-mail(s) Ivan.Lobov@regeneron.com
Organization name Regeneron Pharmaceuticals
Department Regeneron Pharmaceuticals
Street address 777 Old Saw Mill River Road,
City Tarrytown
State/province NY
ZIP/Postal code 10591
Country USA
 
Platform ID GPL13397
Series (1)
GSE28516 Expression analysis of mouse retinas after treatment with VEGF-A or Dll4-Fc.

Data table header descriptions
ID_REF
VALUE lowess normalized natural logarithm of test/reference ratio

Data table
ID_REF VALUE
A_51_P100034 0.29066312
A_51_P100052 -0.011296719
A_51_P100063 0.038296606
A_51_P100065 0.0441527
A_51_P100084 -0.08978512
A_51_P100099 -0.028639881
A_51_P100101 -0.13595243
A_51_P100123
A_51_P100155 -0.12489931
A_51_P100174 -0.008355078
A_51_P100180 -0.18980362
A_51_P100208 -0.037671108
A_51_P100227 0.007912364
A_51_P100238
A_51_P100246 -0.18524194
A_51_P100289 -0.037870668
A_51_P100296 -0.10927029
A_51_P100298 0.16758952
A_51_P100309
A_51_P100312 0.029984828

Total number of rows: 43225

Table truncated, full table size 917 Kbytes.




Supplementary file Size Download File type/resource
GSM706368_R_251534710317_S01_R244k_1_3.txt.gz 15.8 Mb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table

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