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Sample GSM707592 Query DataSets for GSM707592
Status Public on Apr 11, 2012
Title MCF-7, control-2
Sample type RNA
 
Source name MCF-7 cells
Organism Homo sapiens
Characteristics cell line: MCF_7
treatment: serum-free medium x 48 hrs
Treatment protocol Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with serum-free IMEM. Twenty-four hrs later, cells were treated +/- 10 ug/mL insulin. RNA was harvested after 4 or 24 hrs of insulin stimulation.
Growth protocol MCF-7 cells are maintained in IMEM + 10% FBS.
Extracted molecule total RNA
Extraction protocol Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion
Label biotin
Label protocol GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix)
 
Hybridization protocol 15 ug biotinylated cRNA was fragmented and hybridizied to an Affymetrix Genechip Human Genome Plus 2.0-PM array
Scan protocol Hybridized cRNA was detected using streptavidin coupled to phycoerythrin. Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix)
Description Gene expression data from cells treated with serum-free medium x 48 hrs
Data processing Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
 
Submission date Apr 13, 2011
Last update date Apr 11, 2012
Contact name Todd W Miller
E-mail(s) todd.miller@vanderbilt.edu
Organization name Vanderbilt Univ
Department Medicine
Street address 2220 Pierce Ave, 777 PRB
City Nashville
State/province TN
ZIP/Postal code 37174
Country USA
 
Platform ID GPL13158
Series (1)
GSE28580 RNA interference screening identifies the Insulin/IGF-1 receptor pathway as a mechanism of escape from hormone dependence in breast cancer

Data table header descriptions
ID_REF
VALUE rma normalized

Data table
ID_REF VALUE
AFFX-BioB-5_at 6.347499
AFFX-BioB-M_at 7.260844
AFFX-BioB-3_at 6.900898
AFFX-BioC-5_at 7.936264
AFFX-BioC-3_at 8.083911
AFFX-BioDn-5_at 9.270639
AFFX-BioDn-3_at 10.43937
AFFX-CreX-5_at 11.92995
AFFX-CreX-3_at 12.20677
AFFX-DapX-5_at 6.854036
AFFX-DapX-M_at 8.366198
AFFX-DapX-3_at 9.219669
AFFX-LysX-5_at 4.405711
AFFX-LysX-M_at 5.474446
AFFX-LysX-3_at 6.290398
AFFX-PheX-5_at 5.222628
AFFX-PheX-M_at 6.074084
AFFX-PheX-3_at 6.041231
AFFX-ThrX-5_at 3.673646
AFFX-ThrX-M_at 5.556786

Total number of rows: 54715

Table truncated, full table size 1213 Kbytes.




Supplementary file Size Download File type/resource
GSM707592.CEL.gz 1.6 Mb (ftp)(http) CEL
GSM707592.chp.gz 372.3 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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