|
| Status |
Public on Apr 16, 2011 |
| Title |
Th2_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Th2 cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: T helper cell type 2 (Th2)
|
| Growth protocol |
Murine CD4+CD62L+ splenocytes were activated for 5 days with anti-CD3/CD28 in the presence of 10 ng/ml IL-4 and 2 ug/ml anti-IFN-g, followed by 3 days of resting.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Poly(A)+ RNA was isolated from cells using the Oligotex Direct mRNA kit (Qiagen), followed by 1st and 2nd strand synthesis using the JustcDNA kit (Stratagene), followed by sonication, followed by library preparation using the Illumina ChIP-seq sample prep kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Description |
Biological replicate 2 of 2.
|
| Data processing |
Reads were mapped to mm9 with Bowtie -m 1 --best -strata --solexa1.3-quals
|
| |
|
| Submission date |
Apr 15, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Daniel Hebenstreit |
| E-mail(s) |
D.Hebenstreit@warwick.ac.uk
|
| Organization name |
University of Warwick
|
| Street address |
Gibbet Hill Rd
|
| City |
Coventry |
| ZIP/Postal code |
CV47AL |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL11002 |
| Series (1) |
| GSE28666 |
RNA sequencing reveals two major classes of gene expression levels in metazoan cells |
|
| Relations |
| Reanalyzed by |
GSE80797 |
| SRA |
SRX057857 |
| BioSample |
SAMN00259741 |
