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Sample GSM710816 Query DataSets for GSM710816
Status Public on Nov 21, 2011
Title FOLFOX responder training, #1105
Sample type RNA
 
Source name tumor sample of patients with unresectable CRC before mFOLFOX6 therapy
Organism Homo sapiens
Characteristics lesion: Metastasis
location: Peritoneum
mfolfox6: responder
gender: M
type: training
Treatment protocol Surgical specimens were immediately cut into small pieces after resection, snap frozen in liquid nitrogen, and stored at –80°C
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol Experimental procedures for GeneChip(TM) were performed in accordance with the GeneChip Expression Analysis Technical Manual (Affymetrix, Santa Clara, CA), using 3 µg of total RNA.
 
Hybridization protocol Hybridization was performed according to standard protocols for Affymetrix HG-U133 plus 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Data processing The data were analyzed with Affymetrix Power Tools (APT version:1.12.0). The apt-probeset-summarize command were used with -a plier-mm-sketch option.
 
Submission date Apr 19, 2011
Last update date Nov 21, 2011
Contact name Shingo Tsuji
E-mail(s) tsuji@genome.rcast.u-tokyo.ac.jp
Organization name The University of Tokyo
Department Research Center for Advanced Science and Technology
Lab Genome Science div.
Street address 4-6-1 Komaba
City Meguro-ku
State/province Tokyo
ZIP/Postal code 153-8904
Country Japan
 
Platform ID GPL570
Series (1)
GSE28702 CRC samples for FOLFOX therapy prediction

Data table header descriptions
ID_REF probe ID
VALUE APT-calculated Signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 3.57868
AFFX-BioB-M_at 0.00198
AFFX-BioB-3_at 3.33839
AFFX-BioC-5_at 454.23238
AFFX-BioC-3_at 555.38402
AFFX-BioDn-5_at 966.32297
AFFX-BioDn-3_at 1628.25323
AFFX-CreX-5_at 6216.34109
AFFX-CreX-3_at 6689.14149
AFFX-DapX-5_at 21.95451
AFFX-DapX-M_at 13.19910
AFFX-DapX-3_at 5.69264
AFFX-LysX-5_at 2.28541
AFFX-LysX-M_at 20.74487
AFFX-LysX-3_at 22.44138
AFFX-PheX-5_at 24.67723
AFFX-PheX-M_at 31.23344
AFFX-PheX-3_at 22.52709
AFFX-ThrX-5_at 8.04700
AFFX-ThrX-M_at 16.59361

Total number of rows: 54675

Table truncated, full table size 1067 Kbytes.




Supplementary file Size Download File type/resource
GSM710816.CEL.gz 8.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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