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Sample GSM710843 Query DataSets for GSM710843
Status Public on Nov 21, 2011
Title FOLFOX non-responder training, #1111
Sample type RNA
 
Source name tumor sample of patients with unresectable CRC before mFOLFOX6 therapy
Organism Homo sapiens
Characteristics lesion: Metastasis
location: Liver
mfolfox6: non-responder
gender: M
type: training
Treatment protocol Surgical specimens were immediately cut into small pieces after resection, snap frozen in liquid nitrogen, and stored at –80°C
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol Experimental procedures for GeneChip(TM) were performed in accordance with the GeneChip Expression Analysis Technical Manual (Affymetrix, Santa Clara, CA), using 3 µg of total RNA.
 
Hybridization protocol Hybridization was performed according to standard protocols for Affymetrix HG-U133 plus 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Data processing The data were analyzed with Affymetrix Power Tools (APT version:1.12.0). The apt-probeset-summarize command were used with -a plier-mm-sketch option.
 
Submission date Apr 19, 2011
Last update date Nov 21, 2011
Contact name Shingo Tsuji
E-mail(s) tsuji@genome.rcast.u-tokyo.ac.jp
Organization name The University of Tokyo
Department Research Center for Advanced Science and Technology
Lab Genome Science div.
Street address 4-6-1 Komaba
City Meguro-ku
State/province Tokyo
ZIP/Postal code 153-8904
Country Japan
 
Platform ID GPL570
Series (1)
GSE28702 CRC samples for FOLFOX therapy prediction

Data table header descriptions
ID_REF probe ID
VALUE APT-calculated Signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 5.65081
AFFX-BioB-M_at 0.00156
AFFX-BioB-3_at 10.68917
AFFX-BioC-5_at 457.84942
AFFX-BioC-3_at 577.05356
AFFX-BioDn-5_at 1019.20768
AFFX-BioDn-3_at 1821.19480
AFFX-CreX-5_at 6148.96607
AFFX-CreX-3_at 6593.02569
AFFX-DapX-5_at 26.17107
AFFX-DapX-M_at 14.06174
AFFX-DapX-3_at 3.20747
AFFX-LysX-5_at 4.25770
AFFX-LysX-M_at 14.40261
AFFX-LysX-3_at 20.55167
AFFX-PheX-5_at 8.77662
AFFX-PheX-M_at 41.98256
AFFX-PheX-3_at 24.65222
AFFX-ThrX-5_at 6.82902
AFFX-ThrX-M_at 14.95573

Total number of rows: 54675

Table truncated, full table size 1067 Kbytes.




Supplementary file Size Download File type/resource
GSM710843.CEL.gz 8.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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