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Sample GSM710853 Query DataSets for GSM710853
Status Public on Nov 21, 2011
Title FOLFOX non-responder training, #1120
Sample type RNA
 
Source name tumor sample of patients with unresectable CRC before mFOLFOX6 therapy
Organism Homo sapiens
Characteristics lesion: Metastasis
location: Liver
mfolfox6: non-responder
gender: F
type: training
Treatment protocol Surgical specimens were immediately cut into small pieces after resection, snap frozen in liquid nitrogen, and stored at –80°C
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol Experimental procedures for GeneChip(TM) were performed in accordance with the GeneChip Expression Analysis Technical Manual (Affymetrix, Santa Clara, CA), using 3 µg of total RNA.
 
Hybridization protocol Hybridization was performed according to standard protocols for Affymetrix HG-U133 plus 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Data processing The data were analyzed with Affymetrix Power Tools (APT version:1.12.0). The apt-probeset-summarize command were used with -a plier-mm-sketch option.
 
Submission date Apr 19, 2011
Last update date Nov 21, 2011
Contact name Shingo Tsuji
E-mail(s) tsuji@genome.rcast.u-tokyo.ac.jp
Organization name The University of Tokyo
Department Research Center for Advanced Science and Technology
Lab Genome Science div.
Street address 4-6-1 Komaba
City Meguro-ku
State/province Tokyo
ZIP/Postal code 153-8904
Country Japan
 
Platform ID GPL570
Series (1)
GSE28702 CRC samples for FOLFOX therapy prediction

Data table header descriptions
ID_REF probe ID
VALUE APT-calculated Signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 3.31388
AFFX-BioB-M_at 0.00151
AFFX-BioB-3_at 6.14612
AFFX-BioC-5_at 657.17679
AFFX-BioC-3_at 773.91295
AFFX-BioDn-5_at 1347.83561
AFFX-BioDn-3_at 2110.61690
AFFX-CreX-5_at 6503.35132
AFFX-CreX-3_at 7264.12887
AFFX-DapX-5_at 17.75957
AFFX-DapX-M_at 12.56371
AFFX-DapX-3_at 0.49848
AFFX-LysX-5_at 6.74152
AFFX-LysX-M_at 22.02154
AFFX-LysX-3_at 14.59919
AFFX-PheX-5_at 12.28773
AFFX-PheX-M_at 16.40445
AFFX-PheX-3_at 16.16538
AFFX-ThrX-5_at 5.84515
AFFX-ThrX-M_at 11.13768

Total number of rows: 54675

Table truncated, full table size 1069 Kbytes.




Supplementary file Size Download File type/resource
GSM710853.CEL.gz 7.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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