|
Status |
Public on Aug 03, 2012 |
Title |
Myocyte treated with plasma after NH, biological rep6 |
Sample type |
RNA |
|
|
Source name |
Neonatal Myocyte
|
Organism |
Rattus norvegicus |
Characteristics |
tissue: Heart cell type: ventricular myocytes strain: Sprague-Dawley age: 5 days old myocyte culture
|
Treatment protocol |
Cells were treated with Uremic plasma (10%) before and after NHD
|
Growth protocol |
Standard myocyte cell culture
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
|
|
Hybridization protocol |
Affymetrix standard protocol
|
Scan protocol |
GeneChips were scanned using Scanner G3000.
|
Description |
Gene expression data from Myocyte treated with plasma after NHD
|
Data processing |
RMA algorithm in GeneSpring.
|
|
|
Submission date |
Apr 19, 2011 |
Last update date |
Aug 03, 2012 |
Contact name |
Sara Arab |
Organization name |
Toronto General Hospital
|
Department |
Cardiology
|
Lab |
M. Husain
|
Street address |
610 University Avenue
|
City |
Toronto |
State/province |
ONT |
ZIP/Postal code |
M5G 2M9 |
Country |
Canada |
|
|
Platform ID |
GPL6247 |
Series (1) |
GSE28723 |
Effect of Nocturnal Hemodialysis (NHD) on Cardiomyocyte Gene Expression |
|