|
| Status |
Public on Aug 03, 2012 |
| Title |
Myocyte treated with plasma CT, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Neonatal Myocyte
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Heart
cell type: ventricular myocytes
strain: Sprague-Dawley
age: 5 days old myocyte culture
|
| Treatment protocol |
Cells were treated with Uremic plasma (10%) before and after NHD
|
| Growth protocol |
Standard myocyte cell culture
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
GeneChips were scanned using Scanner G3000.
|
| Description |
Gene expression data from Myocyte treated with control plasma
|
| Data processing |
RMA algorithm in GeneSpring.
|
| |
|
| Submission date |
Apr 19, 2011 |
| Last update date |
Aug 03, 2012 |
| Contact name |
Sara Arab |
| Organization name |
Toronto General Hospital
|
| Department |
Cardiology
|
| Lab |
M. Husain
|
| Street address |
610 University Avenue
|
| City |
Toronto |
| State/province |
ONT |
| ZIP/Postal code |
M5G 2M9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE28723 |
Effect of Nocturnal Hemodialysis (NHD) on Cardiomyocyte Gene Expression |
|
