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Sample GSM712691 Query DataSets for GSM712691
Status Public on Dec 31, 2012
Title 4dpf_eye_rep1
Sample type RNA
 
Source name Wild type whole eye on 4dpf
Organism Danio rerio
Characteristics age: 4dpf
tissue: eye
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Qiashredder columns and the RNeasy Minikit (Qiagen, Hilden, Germany) in an RNase-free environment.
Label Biotin
Label protocol Total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
 
Hybridization protocol Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
Scan protocol Scanning was done according to a standard procedure from Affymetrix.
Description Eyes from 4dpf zebrafish embryo, replicate 1
Data processing The signal intensity of the microarray was normalized and summarized using the Bioconductor package, gcrma, using default setting.
 
Submission date Apr 21, 2011
Last update date Dec 31, 2012
Contact name Breandan Kennedy
E-mail(s) brendan.kennedy@ucd.ie
Organization name UCD Conway Institute, University College Dublin
Department UCD School of Biomolecular and Biomedical Science
Lab Kennedy Lab
Street address Belfield
City Dublin
ZIP/Postal code Dublin 4
Country Ireland
 
Platform ID GPL1319
Series (1)
GSE19320 Zebrafish early eye development

Data table header descriptions
ID_REF Affymetrix Probe Set ID
VALUE Log2 signal intensity normalized and summarized using gcrma with default setting

Data table
ID_REF VALUE
AFFX-BioB-3_at 6.18308453668694
AFFX-BioB-5_at 6.69747208643675
AFFX-BioB-M_at 7.71750119422823
AFFX-BioC-3_at 5.34475176584013
AFFX-BioC-5_at 6.55471001385855
AFFX-BioDn-3_at 10.5861467194579
AFFX-BioDn-5_at 10.5352967326234
AFFX-CreX-3_at 13.4829698510596
AFFX-CreX-5_at 12.9478488486314
AFFX-DapX-3_at 8.83916303965563
AFFX-DapX-5_at 3.7770684386875
AFFX-DapX-M_at 8.04321519548414
AFFX-Dr-AB076373-1_at 1.99462354597849
AFFX-Dr-AF292559-1_at 1.78840921281898
AFFX-Dr-AF292559-2_s_at 3.94698375162674
AFFX-Dr-AF292559-3_s_at 3.01272180094912
AFFX-Dr-AF292559-4_s_at 2.0081253410035
AFFX-Dr-AF292560-1_s_at 2.89134644594677
AFFX-Dr-AF298789-1_at 1.92278090124037
AFFX-Dr-AF323980-1_at 2.34593513482349

Total number of rows: 15617

Table truncated, full table size 513 Kbytes.




Supplementary file Size Download File type/resource
GSM712691_S0164F001a.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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