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Sample GSM712692 Query DataSets for GSM712692
Status Public on Dec 31, 2012
Title 4dpf_eye_rep2
Sample type RNA
 
Source name Wild type whole eye on 4dpf
Organism Danio rerio
Characteristics age: 4dpf
tissue: eye
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Qiashredder columns and the RNeasy Minikit (Qiagen, Hilden, Germany) in an RNase-free environment.
Label Biotin
Label protocol Total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
 
Hybridization protocol Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
Scan protocol Scanning was done according to a standard procedure from Affymetrix.
Description Eyes from 4dpf zebrafish embryo, replicate 2
Data processing The signal intensity of the microarray was normalized and summarized using the Bioconductor package, gcrma, using default setting.
 
Submission date Apr 21, 2011
Last update date Dec 31, 2012
Contact name Breandan Kennedy
E-mail(s) brendan.kennedy@ucd.ie
Organization name UCD Conway Institute, University College Dublin
Department UCD School of Biomolecular and Biomedical Science
Lab Kennedy Lab
Street address Belfield
City Dublin
ZIP/Postal code Dublin 4
Country Ireland
 
Platform ID GPL1319
Series (1)
GSE19320 Zebrafish early eye development

Data table header descriptions
ID_REF Affymetrix Probe Set ID
VALUE Log2 signal intensity normalized and summarized using gcrma with default setting

Data table
ID_REF VALUE
AFFX-BioB-3_at 5.84418485721884
AFFX-BioB-5_at 6.30046381627781
AFFX-BioB-M_at 7.2659857226219
AFFX-BioC-3_at 4.87606629170967
AFFX-BioC-5_at 6.08103846603662
AFFX-BioDn-3_at 10.2762351166225
AFFX-BioDn-5_at 9.9855324572267
AFFX-CreX-3_at 13.2983357904399
AFFX-CreX-5_at 12.4463984048009
AFFX-DapX-3_at 7.28333612372468
AFFX-DapX-5_at 2.7464425023463
AFFX-DapX-M_at 6.00762540355101
AFFX-Dr-AB076373-1_at 1.96618475995637
AFFX-Dr-AF292559-1_at 1.75270485701257
AFFX-Dr-AF292559-2_s_at 4.32905170730611
AFFX-Dr-AF292559-3_s_at 3.00164341305077
AFFX-Dr-AF292559-4_s_at 2.01387920934890
AFFX-Dr-AF292560-1_s_at 2.86630578563324
AFFX-Dr-AF298789-1_at 1.89343075958894
AFFX-Dr-AF323980-1_at 2.32047586286731

Total number of rows: 15617

Table truncated, full table size 513 Kbytes.




Supplementary file Size Download File type/resource
GSM712692_S0173F003.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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