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Sample GSM712693 Query DataSets for GSM712693
Status Public on Dec 31, 2012
Title 4dpf_eye_rep3
Sample type RNA
 
Source name Wild type whole eye on 4dpf
Organism Danio rerio
Characteristics age: 4dpf
tissue: eye
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Qiashredder columns and the RNeasy Minikit (Qiagen, Hilden, Germany) in an RNase-free environment.
Label Biotin
Label protocol Total RNA were labeled by Affymetrix GeneChip Two-Cycle Target Labeling protocol.
 
Hybridization protocol Hybridization, washing and staining were done according to a standard procedure from Affymetrix.
Scan protocol Scanning was done according to a standard procedure from Affymetrix.
Description Eyes from 4dpf zebrafish embryo, replicate 3
Data processing The signal intensity of the microarray was normalized and summarized using the Bioconductor package, gcrma, using default setting.
 
Submission date Apr 21, 2011
Last update date Dec 31, 2012
Contact name Breandan Kennedy
E-mail(s) brendan.kennedy@ucd.ie
Organization name UCD Conway Institute, University College Dublin
Department UCD School of Biomolecular and Biomedical Science
Lab Kennedy Lab
Street address Belfield
City Dublin
ZIP/Postal code Dublin 4
Country Ireland
 
Platform ID GPL1319
Series (1)
GSE19320 Zebrafish early eye development

Data table header descriptions
ID_REF Affymetrix Probe Set ID
VALUE Log2 signal intensity normalized and summarized using gcrma with default setting

Data table
ID_REF VALUE
AFFX-BioB-3_at 6.21522888886647
AFFX-BioB-5_at 6.55187355792283
AFFX-BioB-M_at 7.39121533544091
AFFX-BioC-3_at 5.64003897008737
AFFX-BioC-5_at 6.38230608229022
AFFX-BioDn-3_at 10.4184705657858
AFFX-BioDn-5_at 10.3603886933390
AFFX-CreX-3_at 13.6124488922182
AFFX-CreX-5_at 12.6132005583713
AFFX-DapX-3_at 7.3254622430569
AFFX-DapX-5_at 2.73356254517566
AFFX-DapX-M_at 5.709799705272
AFFX-Dr-AB076373-1_at 1.94779893514843
AFFX-Dr-AF292559-1_at 1.73523607746644
AFFX-Dr-AF292559-2_s_at 4.20563819459275
AFFX-Dr-AF292559-3_s_at 2.99314562696703
AFFX-Dr-AF292559-4_s_at 1.99868396631157
AFFX-Dr-AF292560-1_s_at 2.84654827742812
AFFX-Dr-AF298789-1_at 1.87517385838656
AFFX-Dr-AF323980-1_at 2.30059246359187

Total number of rows: 15617

Table truncated, full table size 513 Kbytes.




Supplementary file Size Download File type/resource
GSM712693_S0173F004.CEL.gz 2.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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