|
| Status |
Public on Apr 22, 2011 |
| Title |
WT 1 |
| Sample type |
RNA |
| |
|
| Source name |
Brain of WT mice
|
| Organism |
Mus musculus |
| Characteristics |
gender: Males
strain: C57/B6
age: 3 months old
tissue: Brain
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol, according to manufacturer’s manual, followed by purification with Quigen RNA easy kit.
|
| Label |
Cy3
|
| Label protocol |
Standard protocol recommended by manufacturer Affymetrix
|
| |
|
| Hybridization protocol |
Standard protocol recommended by manufacturer Affymetrix
|
| Scan protocol |
Standard protocol recommended by manufacturer Affymetrix
|
| Data processing |
We used Agilent GeneSpring GX v.11.0 for the analysis of the data. We used Robust Multichip Average (RMA) algorithm as the initial method for chip summarization.
|
| |
|
| Submission date |
Apr 21, 2011 |
| Last update date |
Apr 22, 2011 |
| Contact name |
Sergiy Libert |
| E-mail(s) |
libert@mit.edu
|
| Phone |
617-258-7360
|
| Organization name |
MIT
|
| Street address |
77 Mass ave
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE28790 |
SIRT1 impact on global gene expression in the brain |
|
