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Sample GSM712799 Query DataSets for GSM712799
Status Public on Apr 22, 2011
Title WT 2
Sample type RNA
Source name Brain of WT mice
Organism Mus musculus
Characteristics gender: Males
strain: C57/B6
age: 3 months old
tissue: Brain
Extracted molecule total RNA
Extraction protocol Trizol, according to manufacturer’s manual, followed by purification with Quigen RNA easy kit.
Label Cy3
Label protocol Standard protocol recommended by manufacturer Affymetrix
Hybridization protocol Standard protocol recommended by manufacturer Affymetrix
Scan protocol Standard protocol recommended by manufacturer Affymetrix
Data processing We used Agilent GeneSpring GX v.11.0 for the analysis of the data. We used Robust Multichip Average (RMA) algorithm as the initial method for chip summarization.
Submission date Apr 21, 2011
Last update date Apr 22, 2011
Contact name Sergiy Libert
Phone 617-258-7360
Organization name MIT
Street address 77 Mass ave
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
Platform ID GPL8321
Series (1)
GSE28790 SIRT1 impact on global gene expression in the brain

Data table header descriptions
VALUE log RMA normalized signal

Data table
AFFX-BioB-5_at 0.4265995
AFFX-BioB-M_at 0.33390045
AFFX-BioB-3_at 0.35626507
AFFX-BioC-5_at 0.3621645
AFFX-BioC-3_at 0.3650999
AFFX-BioDn-5_at 0.2598982
AFFX-BioDn-3_at 0.20987606
AFFX-CreX-5_at 0.15616131
AFFX-CreX-3_at 0.08986378
AFFX-DapX-5_at 0.076997995
AFFX-DapX-M_at -0.08286357
AFFX-DapX-3_at -0.07817268
AFFX-LysX-5_at -0.012208939
AFFX-LysX-M_at -0.021667242
AFFX-LysX-3_at 0.058679342
AFFX-PheX-5_at -0.11558318
AFFX-PheX-M_at -0.012988806
AFFX-PheX-3_at -0.18641949
AFFX-ThrX-5_at 0.013181448
AFFX-ThrX-M_at 0.111441135

Total number of rows: 22690

Table truncated, full table size 518 Kbytes.

Supplementary file Size Download File type/resource
GSM712799.CEL.gz 2.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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