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Sample GSM712801 Query DataSets for GSM712801
Status Public on Apr 22, 2011
Title WT 3
Sample type RNA
Source name Brain of WT mice
Organism Mus musculus
Characteristics gender: Males
strain: C57/B6
age: 3 months old
tissue: Brain
Extracted molecule total RNA
Extraction protocol Trizol, according to manufacturer’s manual, followed by purification with Quigen RNA easy kit.
Label Cy3
Label protocol Standard protocol recommended by manufacturer Affymetrix
Hybridization protocol Standard protocol recommended by manufacturer Affymetrix
Scan protocol Standard protocol recommended by manufacturer Affymetrix
Data processing We used Agilent GeneSpring GX v.11.0 for the analysis of the data. We used Robust Multichip Average (RMA) algorithm as the initial method for chip summarization.
Submission date Apr 21, 2011
Last update date Apr 22, 2011
Contact name Sergiy Libert
Phone 617-258-7360
Organization name MIT
Street address 77 Mass ave
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
Platform ID GPL8321
Series (1)
GSE28790 SIRT1 impact on global gene expression in the brain

Data table header descriptions
VALUE log RMA normalized signal

Data table
AFFX-BioB-5_at 0.13179588
AFFX-BioB-M_at 0.064959526
AFFX-BioB-3_at 0.04889393
AFFX-BioC-5_at 0.06993103
AFFX-BioC-3_at 0.049456596
AFFX-BioDn-5_at 0.061151505
AFFX-BioDn-3_at -0.03349209
AFFX-CreX-5_at 0.03673649
AFFX-CreX-3_at 0.003843308
AFFX-DapX-5_at -0.08680558
AFFX-DapX-M_at -0.03239894
AFFX-DapX-3_at 0.043821335
AFFX-LysX-5_at -0.040570736
AFFX-LysX-M_at 0.1608746
AFFX-LysX-3_at 0.018959045
AFFX-PheX-5_at 0.1370368
AFFX-PheX-M_at -0.06897879
AFFX-PheX-3_at -0.0201025
AFFX-ThrX-5_at 0.06530905
AFFX-ThrX-M_at -0.08425975

Total number of rows: 22690

Table truncated, full table size 518 Kbytes.

Supplementary file Size Download File type/resource
GSM712801.CEL.gz 2.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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