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Sample GSM712803 Query DataSets for GSM712803
Status Public on Apr 22, 2011
Title WT 4
Sample type RNA
Source name Brain of WT mice
Organism Mus musculus
Characteristics gender: Males
strain: C57/B6
age: 3 months old
tissue: Brain
Extracted molecule total RNA
Extraction protocol Trizol, according to manufacturer’s manual, followed by purification with Quigen RNA easy kit.
Label Cy3
Label protocol Standard protocol recommended by manufacturer Affymetrix
Hybridization protocol Standard protocol recommended by manufacturer Affymetrix
Scan protocol Standard protocol recommended by manufacturer Affymetrix
Data processing We used Agilent GeneSpring GX v.11.0 for the analysis of the data. We used Robust Multichip Average (RMA) algorithm as the initial method for chip summarization.
Submission date Apr 21, 2011
Last update date Apr 22, 2011
Contact name Sergiy Libert
Phone 617-258-7360
Organization name MIT
Street address 77 Mass ave
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
Platform ID GPL8321
Series (1)
GSE28790 SIRT1 impact on global gene expression in the brain

Data table header descriptions
VALUE log RMA normalized signal

Data table
AFFX-BioB-5_at -0.12273693
AFFX-BioB-M_at -0.106529236
AFFX-BioB-3_at -0.09796238
AFFX-BioC-5_at -0.14118099
AFFX-BioC-3_at -0.1171608
AFFX-BioDn-5_at -0.06115055
AFFX-BioDn-3_at 0.017032623
AFFX-CreX-5_at 0.045886993
AFFX-CreX-3_at 0.049146652
AFFX-DapX-5_at 0.010427713
AFFX-DapX-M_at -0.0684247
AFFX-DapX-3_at 0.19142318
AFFX-LysX-5_at -0.07030845
AFFX-LysX-M_at -0.22288561
AFFX-LysX-3_at -0.06734705
AFFX-PheX-5_at -0.014851332
AFFX-PheX-M_at -0.01551652
AFFX-PheX-3_at -0.053393364
AFFX-ThrX-5_at -0.052556753
AFFX-ThrX-M_at 0.028461695

Total number of rows: 22690

Table truncated, full table size 520 Kbytes.

Supplementary file Size Download File type/resource
GSM712803.CEL.gz 2.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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