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Sample GSM713463 Query DataSets for GSM713463
Status Public on Oct 04, 2011
Title prometaphase NT embryo #27 (4068264030 D)
Sample type RNA
 
Source name zygote
Organism Mus musculus
Characteristics cell type: zygote
Treatment protocol The genome of the mouse zygote or the mouse blastomere was exchanged at mitosis. The extent of reprogramming was determined during the following cell cycle.
Growth protocol mouse embryos were cultured in KSOM
Extracted molecule total RNA
Extraction protocol PicoPure RNA isolation kit (Arcturus)
Label biotin
Label protocol Biotin label introduced according to protocols of the Illumina Total Prep RNA Amplification Kit
 
Hybridization protocol Hybridization according to Illumina protocols on Illumina Sentrix Mouse Expression BeadChip RefSeq 8 v1.1 (GPL6103) or Mouse Expression BeadChip® RefSeq 8 v2.0 (GPL6885) as indicated. Arrays are hybridized, washed, blocked and stained with streptavidin-Cy3. The protocol is WGGEX Direct Hybridization Assay Guide (11322355 A).
Scan protocol According to Illumina protocols with Illumina iScan. Protocols can be found on the illumina web page.
Description NT embryo generated at prometaphase of mitosis with interphase tail tip cells as nuclear donors, 22-24h post transfer

Data processing Data were normalized to average signal, background was subtracted using Illumina GenomeStudio. There are two series presented in a SuperSeries. One series was generated with GPL6103, the other with GPL6885. Normalized data are presented only for probes present in both GPL6103 AND GPL6885. Hybridization duplicates were not used for data analysis, but only for quality control.
 
Submission date Apr 22, 2011
Last update date Oct 04, 2011
Contact name Dieter Egli
E-mail(s) degli@nyscf.org
Phone 617 4968659
Organization name The New York Stem Cell Foundation Research Institute
Department NYSCF
Lab Egli
Street address 3960 Broadway
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platform ID GPL6103
Series (1)
GSE28811 Reprogramming is achieved within a single cell cycle after mouse nuclear transfer

Data table header descriptions
ID_REF
VALUE Average Signal
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1224866 -9.815986 0.874028
ILMN_2632289 -6.03005 0.7076205
ILMN_1223600 -7.790415 0.7869362
ILMN_2756550 5.013 0.2177294
ILMN_2722939 -12.76396 0.9486781
ILMN_2599917 -6.878513 0.7465008
ILMN_1251046 -3.551543 0.5816485
ILMN_1254242 -1.573333 0.4634526
ILMN_2640849 7.383636 0.1664075
ILMN_2755163 -6.728769 0.7402799
ILMN_2646187 -18.29934 0.9937792
ILMN_2741180 -4.312382 0.6127527
ILMN_2621606 -6.359892 0.7216174
ILMN_2655240 2.704237 0.2877139
ILMN_2650700 -5.224699 0.6547434
ILMN_2763729 -6.510593 0.7278383
ILMN_2597810 5.228526 0.2177294
ILMN_1245221 -5.105527 0.6469674
ILMN_2772508 -5.858704 0.6936237
ILMN_1233185 9.432019 0.1368585

Total number of rows: 16075

Table truncated, full table size 478 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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