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Sample GSM71505 Query DataSets for GSM71505
Status Public on Dec 01, 2005
Title MCF7_DMOG_2
Sample type RNA
Source name MCF7, Normoxia 21% Oxygen, DMOG
Organism Homo sapiens
Characteristics human breast carcinoma MCF7 cells
Treatment protocol Cells grown in Normoxia (21% Oxygen) and exposed to DMOG (2 mM) for 16 hours
Growth protocol Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Absolutely RNA RT-PCR Miniprep Kit(Stratagene)
Label Biotin-Labeled cRNA
Label protocol Refer to the Affymetrix Expression Analysis Technical Manual
Hybridization protocol Refer to the Affymetrix Expression Analysis Technical Manual
Scan protocol Refer to the Affymetrix Expression Analysis Technical Manual
Description MCF7 cells grown in Normoxia and exposed to DMOG (dimethyloxalylglycine)
Data processing Normalized using the GCRMA algorithm
Submission date Aug 23, 2005
Last update date Aug 24, 2005
Contact name Ioannis Ragoussis
Organization name University of Oxford
Department The Wellcome Trust Centre for Human Genetics
Lab Genomics
Street address Roosevelt Drive
City Oxford
ZIP/Postal code OX3 7BN
Country United Kingdom
Platform ID GPL96
Series (1)
GSE3188 Hypoxic regulation of gene expression is dominated by the HIF system and can be mimicked by DMOG

Data table header descriptions
VALUE Signal, normalized using the GCRMA algorithm

Data table
1007_s_at 2648.8484
1053_at 212.29553
117_at 127.953926
121_at 198.78198
1255_g_at 4.84333
1294_at 12.923585
1316_at 40.59218
1320_at 8.756636
1405_i_at 19.579134
1431_at 9.937895
1438_at 48.548733
1487_at 198.12222
1494_f_at 13.622898
1598_g_at 143.34172
160020_at 42.73839
1729_at 180.70296
1773_at 15.316862
177_at 10.66257
179_at 318.10287
1861_at 19.09976

Total number of rows: 22283

Table truncated, full table size 447 Kbytes.

Supplementary data files not provided

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