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Sample GSM71507 Query DataSets for GSM71507
Status Public on Dec 01, 2005
Title MCF7_OF_1
Sample type RNA
Source name MCF7, oligofectamine, Hypoxia 1% Oxygen, 16 hours
Organism Homo sapiens
Characteristics human breast carcinoma MCF7 cells
Treatment protocol Cells were exposed to oligofectamine transfection reagent and grown in Hypoxia (1% Oxygen) for 16 hours
Growth protocol Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Absolutely RNA RT-PCR Miniprep Kit(Stratagene)
Label Biotin-Labeled cRNA
Label protocol Refer to the Affymetrix Expression Analysis Technical Manual
Hybridization protocol Refer to the Affymetrix Expression Analysis Technical Manual
Scan protocol Refer to the Affymetrix Expression Analysis Technical Manual
Description MCF7 cells exposed to oligofectamine and grown in Hypoxia
Data processing Normalized using the GCRMA algorithm
Submission date Aug 23, 2005
Last update date Aug 24, 2005
Contact name Ioannis Ragoussis
Organization name University of Oxford
Department The Wellcome Trust Centre for Human Genetics
Lab Genomics
Street address Roosevelt Drive
City Oxford
ZIP/Postal code OX3 7BN
Country United Kingdom
Platform ID GPL570
Series (1)
GSE3188 Hypoxic regulation of gene expression is dominated by the HIF system and can be mimicked by DMOG

Data table header descriptions
VALUE Signal, normalized using the GCRMA algorithm

Data table
1007_s_at 5438.54
1053_at 731.8467
117_at 50.474285
121_at 224.93266
1255_g_at 4.658144
1294_at 11.595094
1316_at 20.481997
1320_at 8.928235
1405_i_at 12.867919
1431_at 7.579922
1438_at 67.88373
1487_at 389.63873
1494_f_at 11.51097
1552256_a_at 2835.6177
1552257_a_at 519.394
1552258_at 6.0211754
1552261_at 8.308918
1552263_at 13.581763
1552264_a_at 204.72012
1552266_at 13.308586

Total number of rows: 54675

Table truncated, full table size 1088 Kbytes.

Supplementary data files not provided

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