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Sample GSM71509 Query DataSets for GSM71509
Status Public on Dec 01, 2005
Title MCF7_OF_2
Sample type RNA
Source name MCF7, oligofectamine, Hypoxia 1% Oxygen, 16 hours
Organism Homo sapiens
Characteristics human breast carcinoma MCF7 cells
Treatment protocol Cells were exposed to oligofectamine transfection reagent and grown in Hypoxia (1% Oxygen) for 16 hours
Growth protocol Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Absolutely RNA RT-PCR Miniprep Kit(Stratagene)
Label Biotin-Labeled cRNA
Label protocol Refer to the Affymetrix Expression Analysis Technical Manual
Hybridization protocol Refer to the Affymetrix Expression Analysis Technical Manual
Scan protocol Refer to the Affymetrix Expression Analysis Technical Manual
Description MCF7 cells exposed to oligofectamine and grown in Hypoxia
Data processing Normalized using the GCRMA algorithm
Submission date Aug 23, 2005
Last update date Aug 24, 2005
Contact name Ioannis Ragoussis
Organization name University of Oxford
Department The Wellcome Trust Centre for Human Genetics
Lab Genomics
Street address Roosevelt Drive
City Oxford
ZIP/Postal code OX3 7BN
Country United Kingdom
Platform ID GPL570
Series (1)
GSE3188 Hypoxic regulation of gene expression is dominated by the HIF system and can be mimicked by DMOG

Data table header descriptions
VALUE Signal, normalized using the GCRMA algorithm

Data table
1007_s_at 3961.3906
1053_at 853.01605
117_at 38.069008
121_at 216.69513
1255_g_at 4.448382
1294_at 10.551982
1316_at 17.865608
1320_at 8.588122
1405_i_at 11.219114
1431_at 6.4775934
1438_at 157.55879
1487_at 314.52524
1494_f_at 10.428033
1552256_a_at 1849.6978
1552257_a_at 677.7134
1552258_at 5.5967336
1552261_at 7.504941
1552263_at 23.599215
1552264_a_at 335.7754
1552266_at 11.92842

Total number of rows: 54675

Table truncated, full table size 1088 Kbytes.

Supplementary data files not provided

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