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Sample GSM71541 Query DataSets for GSM71541
Status Public on Dec 01, 2005
Title MCF7_HIF1_2
Sample type RNA
 
Source name MCF7, HIF-1alpha siRNA, Hypoxia 1% Oxygen, 16 hours
Organism Homo sapiens
Characteristics human breast carcinoma MCF7 cells
Treatment protocol Cells were transfected with HIF-1alpha siRNA and grown in Hypoxia (1% Oxygen) for 16 hours
Growth protocol Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Absolutely RNA RT-PCR Miniprep Kit(Stratagene)
Label Biotin-Labeled cRNA
Label protocol Refer to the Affymetrix Expression Analysis Technical Manual
 
Hybridization protocol Refer to the Affymetrix Expression Analysis Technical Manual
Scan protocol Refer to the Affymetrix Expression Analysis Technical Manual
Description MCF7 cells transfected with HIF-1alpha siRNA and grown in Hypoxia
Data processing Normalized using the GCRMA algorithm
 
Submission date Aug 23, 2005
Last update date Aug 24, 2005
Contact name Ioannis Ragoussis
E-mail(s) ioannis.ragoussis@well.ox.ac.uk
URL http://www.well.ox.ac.uk/genomics/index.shtml
Organization name University of Oxford
Department The Wellcome Trust Centre for Human Genetics
Lab Genomics
Street address Roosevelt Drive
City Oxford
ZIP/Postal code OX3 7BN
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE3188 Hypoxic regulation of gene expression is dominated by the HIF system and can be mimicked by DMOG

Data table header descriptions
ID_REF
VALUE Signal, normalized using the GCRMA algorithm

Data table
ID_REF VALUE
1007_s_at 2675.01
1053_at 870.56805
117_at 34.279877
121_at 206.23213
1255_g_at 4.496108
1294_at 10.571553
1316_at 18.291304
1320_at 8.870517
1405_i_at 11.7395735
1431_at 6.7721834
1438_at 110.36895
1487_at 468.13223
1494_f_at 10.41849
1552256_a_at 1249.431
1552257_a_at 607.9875
1552258_at 5.67698
1552261_at 6.431788
1552263_at 21.48462
1552264_a_at 339.14523
1552266_at 12.241932

Total number of rows: 54675

Table truncated, full table size 1088 Kbytes.




Supplementary data files not provided

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