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Sample GSM71547 Query DataSets for GSM71547
Status Public on Dec 01, 2005
Title MCF7_HIF12_2
Sample type RNA
 
Source name MCF7, HIF-1alpha and HIF-2alpha siRNA, Hypoxia 1% Oxygen, 16 hours
Organism Homo sapiens
Characteristics human breast carcinoma MCF7 cells
Treatment protocol Cells were transfected with HIF-1alpha and HIF-2alpha siRNA and grown in Hypoxia (1% Oxygen) for 16 hours
Growth protocol Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Absolutely RNA RT-PCR Miniprep Kit(Stratagene)
Label Biotin-Labeled cRNA
Label protocol Refer to the Affymetrix Expression Analysis Technical Manual
 
Hybridization protocol Refer to the Affymetrix Expression Analysis Technical Manual
Scan protocol Refer to the Affymetrix Expression Analysis Technical Manual
Description MCF7 cells transfected with HIF-1alpha and HIF-2alpha siRNA and grown in Hypoxia
Data processing Normalized using the GCRMA algorithm
 
Submission date Aug 23, 2005
Last update date Aug 24, 2005
Contact name Ioannis Ragoussis
E-mail(s) ioannis.ragoussis@well.ox.ac.uk
URL http://www.well.ox.ac.uk/genomics/index.shtml
Organization name University of Oxford
Department The Wellcome Trust Centre for Human Genetics
Lab Genomics
Street address Roosevelt Drive
City Oxford
ZIP/Postal code OX3 7BN
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE3188 Hypoxic regulation of gene expression is dominated by the HIF system and can be mimicked by DMOG

Data table header descriptions
ID_REF
VALUE Signal, normalized using the GCRMA algorithm

Data table
ID_REF VALUE
1007_s_at 2798.0125
1053_at 761.0455
117_at 49.44774
121_at 240.40227
1255_g_at 4.5830483
1294_at 12.247937
1316_at 21.548708
1320_at 9.023534
1405_i_at 13.079054
1431_at 7.023058
1438_at 129.90323
1487_at 483.87314
1494_f_at 10.817993
1552256_a_at 1031.8582
1552257_a_at 591.6947
1552258_at 6.761192
1552261_at 7.825752
1552263_at 16.808756
1552264_a_at 334.88382
1552266_at 13.045451

Total number of rows: 54675

Table truncated, full table size 1088 Kbytes.




Supplementary data files not provided

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