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Sample GSM71548 Query DataSets for GSM71548
Status Public on Dec 01, 2005
Title MCF7_HIF12_3
Sample type RNA
 
Source name MCF7, HIF-1alpha and HIF-2alpha siRNA, Hypoxia 1% Oxygen, 16 hours
Organism Homo sapiens
Characteristics human breast carcinoma MCF7 cells
Treatment protocol Cells were transfected with HIF-1alpha and HIF-2alpha siRNA and grown in Hypoxia (1% Oxygen) for 16 hours
Growth protocol Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Absolutely RNA RT-PCR Miniprep Kit(Stratagene)
Label Biotin-Labeled cRNA
Label protocol Refer to the Affymetrix Expression Analysis Technical Manual
 
Hybridization protocol Refer to the Affymetrix Expression Analysis Technical Manual
Scan protocol Refer to the Affymetrix Expression Analysis Technical Manual
Description MCF7 cells transfected with HIF-1alpha and HIF-2alpha siRNA and grown in Hypoxia
Data processing Normalized using the GCRMA algorithm
 
Submission date Aug 23, 2005
Last update date Aug 24, 2005
Contact name Ioannis Ragoussis
E-mail(s) ioannis.ragoussis@well.ox.ac.uk
URL http://www.well.ox.ac.uk/genomics/index.shtml
Organization name University of Oxford
Department The Wellcome Trust Centre for Human Genetics
Lab Genomics
Street address Roosevelt Drive
City Oxford
ZIP/Postal code OX3 7BN
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE3188 Hypoxic regulation of gene expression is dominated by the HIF system and can be mimicked by DMOG

Data table header descriptions
ID_REF
VALUE Signal, normalized using the GCRMA algorithm

Data table
ID_REF VALUE
1007_s_at 2335.025
1053_at 769.23773
117_at 40.462612
121_at 225.48193
1255_g_at 4.41452
1294_at 10.495656
1316_at 15.17463
1320_at 18.314798
1405_i_at 10.80998
1431_at 6.550803
1438_at 115.49344
1487_at 451.0002
1494_f_at 11.080799
1552256_a_at 1449.9128
1552257_a_at 649.4778
1552258_at 5.556855
1552261_at 7.5495768
1552263_at 19.740295
1552264_a_at 481.7822
1552266_at 13.093886

Total number of rows: 54675

Table truncated, full table size 1088 Kbytes.




Supplementary data files not provided

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