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Sample GSM71605 Query DataSets for GSM71605
Status Public on Dec 01, 2005
Title MCF7_Normoxia_2_Illumina
Sample type RNA
Source name MCF7, Normoxia 21% Oxygen
Organism Homo sapiens
Characteristics human breast carcinoma MCF7 cells
Treatment protocol Normoxia (21% oxygen)
Growth protocol Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Absolutely RNA RT-PCR Miniprep Kit(Stratagene)
Label Biotin-Labeled cRNA
Label protocol Refer to the Illumina Gene Expression System Manual
Hybridization protocol Refer to the Illumina Gene Expression System Manual
Scan protocol Refer to the Illumina Gene Expression System Manual
Description MCF7 cells grown in Normoxia
Data processing Background was subtracted using the Illumina Studio, intensities were shifted to a minimum value of 20 and quantile normalization was performed using functions within R.
Submission date Aug 23, 2005
Last update date Aug 24, 2005
Contact name Ioannis Ragoussis
Organization name University of Oxford
Department The Wellcome Trust Centre for Human Genetics
Lab Genomics
Street address Roosevelt Drive
City Oxford
ZIP/Postal code OX3 7BN
Country United Kingdom
Platform ID GPL2507
Series (1)
GSE3188 Hypoxic regulation of gene expression is dominated by the HIF system and can be mimicked by DMOG

Data table header descriptions
VALUE Signal, quantile normalization performed using functions within R

Data table
GI_10047089-S 6.353506974
GI_10047091-S 7.214302438
GI_10047093-S 10.04015654
GI_10047099-S 6.741376253
GI_10047103-S 11.16389839
GI_10047105-S 6.789016486
GI_10047121-S 6.097147584
GI_10047123-S 11.76734077
GI_10047133-A 6.639520874
GI_10047133-I 6.462550025
GI_10092578-S 6.742724106
GI_10092585-S 7.466300239
GI_10092596-S 8.938040963
GI_10092600-S 10.78548971
GI_10092602-S 6.285556336
GI_10092603-S 7.138640794
GI_10092611-A 9.931162035
GI_10092616-S 8.21357918
GI_10092618-S 11.82350555
GI_10092638-S 7.617431912

Total number of rows: 47293

Table truncated, full table size 1140 Kbytes.

Supplementary data files not provided

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