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Sample GSM71609 Query DataSets for GSM71609
Status Public on Dec 01, 2005
Title MCF7_Hypoxia_2_Illumina
Sample type RNA
Source name MCF7, Hypoxia 1% Oxygen, 16 hours
Organism Homo sapiens
Characteristics human breast carcinoma MCF7 cells
Treatment protocol Hypoxia 1% Oxygen, 16 hours in an Invivo2 Hypoxia Workstation
Growth protocol Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Absolutely RNA RT-PCR Miniprep Kit(Stratagene)
Label Biotin-Labeled cRNA
Label protocol Refer to the Illumina Gene Expression System Manual
Hybridization protocol Refer to the Illumina Gene Expression System Manual
Scan protocol Refer to the Illumina Gene Expression System Manual
Description MCF7 cells grown in Hypoxia
Data processing Background was subtracted using the Illumina Studio, intensities were shifted to a minimum value of 20 and quantile normalization was performed using functions within R.
Submission date Aug 23, 2005
Last update date Aug 24, 2005
Contact name Ioannis Ragoussis
Organization name University of Oxford
Department The Wellcome Trust Centre for Human Genetics
Lab Genomics
Street address Roosevelt Drive
City Oxford
ZIP/Postal code OX3 7BN
Country United Kingdom
Platform ID GPL2507
Series (1)
GSE3188 Hypoxic regulation of gene expression is dominated by the HIF system and can be mimicked by DMOG

Data table header descriptions
VALUE Signal, quantile normalization performed using functions within R

Data table
GI_10047089-S 6.32127859
GI_10047091-S 7.396614944
GI_10047093-S 9.179469865
GI_10047099-S 6.845762721
GI_10047103-S 11.22318868
GI_10047105-S 6.696947254
GI_10047121-S 6.361450716
GI_10047123-S 11.72968378
GI_10047133-A 6.637347773
GI_10047133-I 6.619852961
GI_10092578-S 6.327608822
GI_10092585-S 7.707904035
GI_10092596-S 8.077769638
GI_10092600-S 10.57209926
GI_10092602-S 6.772645153
GI_10092603-S 6.917679811
GI_10092611-A 9.812700544
GI_10092616-S 8.292418462
GI_10092618-S 12.11219904
GI_10092638-S 7.834424214

Total number of rows: 47293

Table truncated, full table size 1140 Kbytes.

Supplementary data files not provided

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