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Sample GSM71611 Query DataSets for GSM71611
Status Public on Dec 01, 2005
Title MCF7_Hypoxia_3_Illumina
Sample type RNA
 
Source name MCF7, Hypoxia 1% Oxygen, 16 hours
Organism Homo sapiens
Characteristics human breast carcinoma MCF7 cells
Treatment protocol Hypoxia 1% Oxygen, 16 hours in an Invivo2 Hypoxia Workstation
Growth protocol Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Absolutely RNA RT-PCR Miniprep Kit(Stratagene)
Label Biotin-Labeled cRNA
Label protocol Refer to the Illumina Gene Expression System Manual
 
Hybridization protocol Refer to the Illumina Gene Expression System Manual
Scan protocol Refer to the Illumina Gene Expression System Manual
Description MCF7 cells grown in Hypoxia
Data processing Background was subtracted using the Illumina Studio, intensities were shifted to a minimum value of 20 and quantile normalization was performed using functions within R.
 
Submission date Aug 23, 2005
Last update date Aug 24, 2005
Contact name Ioannis Ragoussis
E-mail(s) ioannis.ragoussis@well.ox.ac.uk
URL http://www.well.ox.ac.uk/genomics/index.shtml
Organization name University of Oxford
Department The Wellcome Trust Centre for Human Genetics
Lab Genomics
Street address Roosevelt Drive
City Oxford
ZIP/Postal code OX3 7BN
Country United Kingdom
 
Platform ID GPL2507
Series (1)
GSE3188 Hypoxic regulation of gene expression is dominated by the HIF system and can be mimicked by DMOG

Data table header descriptions
ID_REF
VALUE Signal, quantile normalization performed using functions within R

Data table
ID_REF VALUE
GI_10047089-S 6.128561309
GI_10047091-S 6.988285642
GI_10047093-S 9.470932908
GI_10047099-S 6.739359534
GI_10047103-S 11.18367674
GI_10047105-S 7.19731307
GI_10047121-S 6.367603471
GI_10047123-S 11.8258898
GI_10047133-A 6.448536999
GI_10047133-I 6.834481261
GI_10092578-S 6.090802873
GI_10092585-S 8.21321625
GI_10092596-S 8.559203326
GI_10092600-S 10.65866505
GI_10092602-S 6.681581431
GI_10092603-S 6.717629374
GI_10092611-A 9.888745689
GI_10092616-S 8.440038361
GI_10092618-S 11.96103259
GI_10092638-S 8.605153029

Total number of rows: 47293

Table truncated, full table size 1139 Kbytes.




Supplementary data files not provided

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