NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM71615 Query DataSets for GSM71615
Status Public on Dec 01, 2005
Title MCF7_DMOG_2_Illumina
Sample type RNA
 
Source name MCF7, Normoxia 21% Oxygen, DMOG
Organism Homo sapiens
Characteristics human breast carcinoma MCF7 cells
Treatment protocol Cells grown in Normoxia (21% Oxygen) and exposed to DMOG (2 mM) for 16 hours
Growth protocol Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Absolutely RNA RT-PCR Miniprep Kit(Stratagene)
Label Biotin-Labeled cRNA
Label protocol Refer to the Illumina Gene Expression System Manual
 
Hybridization protocol Refer to the Illumina Gene Expression System Manual
Scan protocol Refer to the Illumina Gene Expression System Manual
Description MCF7 cells grown in Normoxia and exposed to DMOG (dimethyloxalylglycine)
Data processing Background was subtracted using the Illumina Studio, intensities were shifted to a minimum value of 20 and quantile normalization was performed using functions within R.
 
Submission date Aug 23, 2005
Last update date Aug 24, 2005
Contact name Ioannis Ragoussis
E-mail(s) ioannis.ragoussis@well.ox.ac.uk
URL http://www.well.ox.ac.uk/genomics/index.shtml
Organization name University of Oxford
Department The Wellcome Trust Centre for Human Genetics
Lab Genomics
Street address Roosevelt Drive
City Oxford
ZIP/Postal code OX3 7BN
Country United Kingdom
 
Platform ID GPL2507
Series (1)
GSE3188 Hypoxic regulation of gene expression is dominated by the HIF system and can be mimicked by DMOG

Data table header descriptions
ID_REF
VALUE Signal, quantile normalization performed using functions within R

Data table
ID_REF VALUE
GI_10047089-S 5.885195674
GI_10047091-S 7.299294628
GI_10047093-S 9.141515338
GI_10047099-S 7.14520961
GI_10047103-S 10.98812045
GI_10047105-S 6.487741296
GI_10047121-S 6.487741296
GI_10047123-S 11.47711399
GI_10047133-A 6.840119867
GI_10047133-I 6.542996771
GI_10092578-S 6.309000994
GI_10092585-S 8.044347543
GI_10092596-S 8.757964815
GI_10092600-S 10.5967423
GI_10092602-S 6.714203642
GI_10092603-S 7.069463856
GI_10092611-A 9.806606937
GI_10092616-S 8.767472097
GI_10092618-S 11.85900738
GI_10092638-S 8.598120954

Total number of rows: 47293

Table truncated, full table size 1140 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap