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Sample GSM71616 Query DataSets for GSM71616
Status Public on Dec 01, 2005
Title MCF7_DMOG_3_Illumina
Sample type RNA
Source name MCF7, Normoxia 21% Oxygen, DMOG
Organism Homo sapiens
Characteristics human breast carcinoma MCF7 cells
Treatment protocol Cells grown in Normoxia (21% Oxygen) and exposed to DMOG (2 mM) for 16 hours
Growth protocol Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Absolutely RNA RT-PCR Miniprep Kit(Stratagene)
Label Biotin-Labeled cRNA
Label protocol Refer to the Illumina Gene Expression System Manual
Hybridization protocol Refer to the Illumina Gene Expression System Manual
Scan protocol Refer to the Illumina Gene Expression System Manual
Description MCF7 cells grown in Normoxia and exposed to DMOG (dimethyloxalylglycine)
Data processing Background was subtracted using the Illumina Studio, intensities were shifted to a minimum value of 20 and quantile normalization was performed using functions within R.
Submission date Aug 23, 2005
Last update date Aug 24, 2005
Contact name Ioannis Ragoussis
Organization name University of Oxford
Department The Wellcome Trust Centre for Human Genetics
Lab Genomics
Street address Roosevelt Drive
City Oxford
ZIP/Postal code OX3 7BN
Country United Kingdom
Platform ID GPL2507
Series (1)
GSE3188 Hypoxic regulation of gene expression is dominated by the HIF system and can be mimicked by DMOG

Data table header descriptions
VALUE Signal, quantile normalization performed using functions within R

Data table
GI_10047089-S 6.357935963
GI_10047091-S 7.139665636
GI_10047093-S 9.403462666
GI_10047099-S 6.893705417
GI_10047103-S 11.08518094
GI_10047105-S 7.092682417
GI_10047121-S 6.119188934
GI_10047123-S 11.41281044
GI_10047133-A 6.951114062
GI_10047133-I 6.601399391
GI_10092578-S 6.385901532
GI_10092585-S 7.638720895
GI_10092596-S 8.422273202
GI_10092600-S 10.50797292
GI_10092602-S 6.866878411
GI_10092603-S 7.257438377
GI_10092611-A 10.02813088
GI_10092616-S 8.382190381
GI_10092618-S 11.92057713
GI_10092638-S 8.490444761

Total number of rows: 47293

Table truncated, full table size 1140 Kbytes.

Supplementary data files not provided

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