|
| Status |
Public on Dec 21, 2012 |
| Title |
amyloid-beta + C1q treated neurons, replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
immature primary cortical neurons
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell type: immature primary cortical neurons
treatment: fibrillar amyloid beta and C1q
|
| Treatment protocol |
Neurons are treated with 5 μM amyloid beta and/or 10 nM human purified C1q
|
| Growth protocol |
primary cortical neurons are isolated form E16 rat embryos and maintained for 3 days in Neurobasal media supplement with N2 before stimulation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using QIAGEN Rneasy extraction kit according to manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (performed by the personnel of the microarray facility at University of California Irvine)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Rat Gene 1.0 ST Array. GeneChips were washed and stained in the GeneChip Fluidics Stations 450
|
| Scan protocol |
GeneChips were scanned using the GeneChip 3000 Scanner 7G with Autoloader
|
| Description |
0908F-02_CIQ + AB 1
gene expression data form neurons treated for 3h with fibrillar amyloid beta and C1q
|
| Data processing |
The data were analyzed with JMP genomics (v5.0) using the basic expression workflow and median signal intensity as normalization method.
Processed data (probe-level) is available on Series record.
|
| |
|
| Submission date |
Apr 28, 2011 |
| Last update date |
Dec 22, 2012 |
| Contact name |
Marie Emeline Benoit |
| E-mail(s) |
mbenoit@uci.edu
|
| Organization name |
UC Irvine
|
| Department |
Molecular Biology and Biochemistry
|
| Lab |
Tenner lab
|
| Street address |
2419 McGaugh Hall
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92697 |
| Country |
USA |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE28886 |
Modulation of gene expression by complement protein C1q in amyloid-beta injured neurons |
|
