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Sample GSM71659 Query DataSets for GSM71659
Status Public on Dec 01, 2005
Title MCF7_OF_4_Illumina
Sample type RNA
 
Source name MCF7, oligofectamine, Hypoxia 1% Oxygen, 16 hours
Organism Homo sapiens
Characteristics human breast carcinoma MCF7 cells
Treatment protocol Cells were exposed to oligofectamine transfection reagent and grown in Hypoxia (1% Oxygen) for 16 hours
Growth protocol Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Absolutely RNA RT-PCR Miniprep Kit (Stratagene)
Label Biotin-Labeled cRNA
Label protocol Refer to the Illumina Gene Expression System Manual
 
Hybridization protocol Refer to the Illumina Gene Expression System Manual
Scan protocol Refer to the Illumina Gene Expression System Manual
Description MCF7 cells exposed to oligofectamine and grown in Hypoxia
Data processing Background was subtracted using the Illumina Studio, intensities were shifted to a minimum value of 20 and quantile normalization was performed using functions within R
 
Submission date Aug 24, 2005
Last update date Aug 24, 2005
Contact name Ioannis Ragoussis
E-mail(s) ioannis.ragoussis@well.ox.ac.uk
URL http://www.well.ox.ac.uk/genomics/index.shtml
Organization name University of Oxford
Department The Wellcome Trust Centre for Human Genetics
Lab Genomics
Street address Roosevelt Drive
City Oxford
ZIP/Postal code OX3 7BN
Country United Kingdom
 
Platform ID GPL2507
Series (1)
GSE3188 Hypoxic regulation of gene expression is dominated by the HIF system and can be mimicked by DMOG

Data table header descriptions
ID_REF
VALUE Signal, quantile normalization performed using functions within R

Data table
ID_REF VALUE
GI_10047089-S 38.14996723
GI_10047091-S 53.34997657
GI_10047093-S 552.961174
GI_10047099-S 62.39927884
GI_10047103-S 1739.635548
GI_10047105-S 50.14997508
GI_10047121-S 36.74996599
GI_10047123-S 1377.15567
GI_10047133-A 46
GI_10047133-I 44.2
GI_10092578-S 36.2
GI_10092585-S 57.3
GI_10092596-S 110.8350125
GI_10092600-S 863.8018523
GI_10092602-S 49.04997452
GI_10092603-S 52.24997608
GI_10092611-A 432.9211553
GI_10092616-S 88.15554435
GI_10092618-S 787.6470276
GI_10092638-S 76.62519168

Total number of rows: 47293

Table truncated, full table size 991 Kbytes.




Supplementary data files not provided

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