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Sample GSM71662 Query DataSets for GSM71662
Status Public on Dec 01, 2005
Title MCF7_HIF1_4_Illumina
Sample type RNA
 
Source name MCF7, HIF-1alpha siRNA, Hypoxia 1% Oxygen, 16 hours
Organism Homo sapiens
Characteristics human breast carcinoma MCF7 cells
Treatment protocol Cells were transfected with HIF-1alpha siRNA and grown in Hypoxia (1% Oxygen) for 16 hours
Growth protocol Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Absolutely RNA RT-PCR Miniprep Kit (Stratagene)
Label Biotin-Labeled cRNA
Label protocol Refer to the Illumina Gene Expression System Manual
 
Hybridization protocol Refer to the Illumina Gene Expression System Manual
Scan protocol Refer to the Illumina Gene Expression System Manual
Description MCF7 cells transfected with HIF-1alpha siRNA and grown in Hypoxia
Data processing Background was subtracted using the Illumina Studio, intensities were shifted to a minimum value of 20 and quantile normalization was performed using functions within R
 
Submission date Aug 24, 2005
Last update date Aug 24, 2005
Contact name Ioannis Ragoussis
E-mail(s) ioannis.ragoussis@well.ox.ac.uk
URL http://www.well.ox.ac.uk/genomics/index.shtml
Organization name University of Oxford
Department The Wellcome Trust Centre for Human Genetics
Lab Genomics
Street address Roosevelt Drive
City Oxford
ZIP/Postal code OX3 7BN
Country United Kingdom
 
Platform ID GPL2507
Series (1)
GSE3188 Hypoxic regulation of gene expression is dominated by the HIF system and can be mimicked by DMOG

Data table header descriptions
ID_REF
VALUE Signal, quantile normalization performed using functions within R

Data table
ID_REF VALUE
GI_10047089-S 48
GI_10047091-S 53.2
GI_10047093-S 594.3867428
GI_10047099-S 78.52057055
GI_10047103-S 1977.357398
GI_10047105-S 44.9
GI_10047121-S 39
GI_10047123-S 1342.789522
GI_10047133-A 51.1
GI_10047133-I 48.3
GI_10092578-S 41.64996999
GI_10092585-S 61.39967427
GI_10092596-S 180.4808023
GI_10092600-S 1107.626783
GI_10092602-S 42.1
GI_10092603-S 42.2
GI_10092611-A 409.0890368
GI_10092616-S 98.69697891
GI_10092618-S 1316.034605
GI_10092638-S 115.8957446

Total number of rows: 47293

Table truncated, full table size 991 Kbytes.




Supplementary data files not provided

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