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Sample GSM71663 Query DataSets for GSM71663
Status Public on Dec 01, 2005
Title MCF7_HIF1_5_Illumina
Sample type RNA
 
Source name MCF7, HIF-1alpha siRNA, Hypoxia 1% Oxygen, 16 hours
Organism Homo sapiens
Characteristics human breast carcinoma MCF7 cells
Treatment protocol Cells were transfected with HIF-1alpha siRNA and grown in Hypoxia (1% Oxygen) for 16 hours
Growth protocol Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Absolutely RNA RT-PCR Miniprep Kit (Stratagene)
Label Biotin-Labeled cRNA
Label protocol Refer to the Illumina Gene Expression System Manual
 
Hybridization protocol Refer to the Illumina Gene Expression System Manual
Scan protocol Refer to the Illumina Gene Expression System Manual
Description MCF7 cells transfected with HIF-1alpha siRNA and grown in Hypoxia
Data processing Background was subtracted using the Illumina Studio, intensities were shifted to a minimum value of 20 and quantile normalization was performed using functions within R
 
Submission date Aug 24, 2005
Last update date Aug 24, 2005
Contact name Ioannis Ragoussis
E-mail(s) ioannis.ragoussis@well.ox.ac.uk
URL http://www.well.ox.ac.uk/genomics/index.shtml
Organization name University of Oxford
Department The Wellcome Trust Centre for Human Genetics
Lab Genomics
Street address Roosevelt Drive
City Oxford
ZIP/Postal code OX3 7BN
Country United Kingdom
 
Platform ID GPL2507
Series (1)
GSE3188 Hypoxic regulation of gene expression is dominated by the HIF system and can be mimicked by DMOG

Data table header descriptions
ID_REF
VALUE Signal, quantile normalization performed using functions within R

Data table
ID_REF VALUE
GI_10047089-S 39.34996823
GI_10047091-S 60.74981481
GI_10047093-S 600.8178815
GI_10047099-S 60.64981451
GI_10047103-S 1757.1665
GI_10047105-S 52.94997639
GI_10047121-S 38.1
GI_10047123-S 1595.564129
GI_10047133-A 44.2
GI_10047133-I 51.6
GI_10092578-S 37.04996626
GI_10092585-S 56.8
GI_10092596-S 165.8273198
GI_10092600-S 1201.994759
GI_10092602-S 46.94997338
GI_10092603-S 45.6
GI_10092611-A 450.3960035
GI_10092616-S 94.21809186
GI_10092618-S 1207.883066
GI_10092638-S 104.4988038

Total number of rows: 47293

Table truncated, full table size 983 Kbytes.




Supplementary data files not provided

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