|
| Status |
Public on Apr 30, 2011 |
| Title |
MIA on day 21, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
monoiodoacetate induced arthritis on day 21 post-injection, biological rep2
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
age: 12 ~ 14 weeks
tissue: articular cartilage
agent: monoiodoacetate
time: day 21 post-injection
|
| Treatment protocol |
Monoiodoacetate (MIA, 2 mg) dissolved in 50 ul of saline was intra-articularly injected into a knee of hinder limbs. Articular cartilage in the distal end of femur was exercised after 5, 9 or 21 days post-injection. Saline injected rats were used as sham controls.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 300 ng of total RNA according to Affymetrix's protocols.
|
| |
|
| Hybridization protocol |
2ug of labeled and fragmented DNA was hybridized at 45C for 17 hours at 60rpm. GeneChips were washed and stained on the Affymetrix Fluidics Station 450 according to the manufacturers protocol.
|
| Scan protocol |
GeneChips were scanned with the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
JN-5.CEL
Gene expression data from articular cartilage in the distal end of femur from MIA-induced arthritic knees on day 21 post-injection
|
| Data processing |
The data were analyzed by Partek Genomics Suite version 17 using GC-RMA normalization.
|
| |
|
| Submission date |
Apr 29, 2011 |
| Last update date |
Apr 30, 2011 |
| Contact name |
Jin Nam |
| Organization name |
The Ohio State University
|
| Street address |
305 W. 12th Ave., 4010 Postle Hall
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE28958 |
Expression data from monoiodoacetate-induced arthritis in a rat model |
|
