|
| Status |
Public on May 01, 2011 |
| Title |
Combination treated_4 |
| Sample type |
RNA |
| |
|
| Source name |
Combination_4h_treated
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
age: 5-8 weeks
|
| Treatment protocol |
C57BL/6J mice were placed, unrestrained, in a aerosolization chamber and inhalationally exposed to 20 minute treatment with an 8 ml volume of PBS (sham), Pam2CSK4 10 ug/ml, ODN 2395 20 ug/ml, or the combination.
|
| Growth protocol |
C57BL/6J mice were 5-8 weeks old
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA extracted using Qiagen Rneasy per manufacturers instructions. cRNA amplification using Ambion total prep kit per manufacturers instructions.
|
| Label |
Cy3
|
| Label protocol |
cRNA was hybridized to BeadChips prior to labeling with Cy3-Streptavidin per Illumina instructions.
|
| |
|
| Hybridization protocol |
cRNA was hybridized to BeadChips per Illumina instructions.
|
| Scan protocol |
Chips were scanned using level 3 signal using a BeadStation 500 scanner.
|
| Description |
5511141053_E
|
| Data processing |
Data was processed using Illumina BeadStudio v3. Matrix tables were generated both without normalization and with rank invariant normalization, using sham group as the reference group.
|
| |
|
| Submission date |
Apr 30, 2011 |
| Last update date |
May 01, 2011 |
| Contact name |
Scott E Evans |
| E-mail(s) |
seevans@mdanderson.org
|
| Organization name |
University of Texas-M.D. Anderson Cancer Center
|
| Department |
Pulmonary Medicine - Box 403
|
| Street address |
1400 Holcombe Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE28994 |
Gene expression in mouse lung homogenates treated with synthetic TLR ligands to induce resistance |
|
