NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM718389 Query DataSets for GSM718389
Status Public on May 01, 2011
Title ODN2395 treated_6
Sample type RNA
 
Source name ODN2395_4h_treated
Organism Mus musculus
Characteristics strain: C57BL/6J
age: 5-8 weeks
Treatment protocol C57BL/6J mice were placed, unrestrained, in a aerosolization chamber and inhalationally exposed to 20 minute treatment with an 8 ml volume of PBS (sham), Pam2CSK4 10 ug/ml, ODN 2395 20 ug/ml, or the combination.
Growth protocol C57BL/6J mice were 5-8 weeks old
Extracted molecule total RNA
Extraction protocol total RNA extracted using Qiagen Rneasy per manufacturers instructions. cRNA amplification using Ambion total prep kit per manufacturers instructions.
Label Cy3
Label protocol cRNA was hybridized to BeadChips prior to labeling with Cy3-Streptavidin per Illumina instructions.
 
Hybridization protocol cRNA was hybridized to BeadChips per Illumina instructions.
Scan protocol Chips were scanned using level 3 signal using a BeadStation 500 scanner.
Description 5511141059_E
Data processing Data was processed using Illumina BeadStudio v3. Matrix tables were generated both without normalization and with rank invariant normalization, using sham group as the reference group.
 
Submission date Apr 30, 2011
Last update date May 01, 2011
Contact name Scott E Evans
E-mail(s) seevans@mdanderson.org
Organization name University of Texas-M.D. Anderson Cancer Center
Department Pulmonary Medicine - Box 403
Street address 1400 Holcombe Blvd
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6885
Series (1)
GSE28994 Gene expression in mouse lung homogenates treated with synthetic TLR ligands to induce resistance

Data table header descriptions
ID_REF
VALUE Values reported were derived using Illumina BeadStudio v3 and are relative signal intensity normalized to background.
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_2896528 3670.575 47 123.6191 0
ILMN_2721178 1018.524 38 44.40987 0
ILMN_3033922 949.4979 41 44.46275 0
ILMN_3092673 6065.58 51 262.9545 0
ILMN_2816356 70.32653 34 10.97404 0
ILMN_2808939 861.9221 46 49.67702 0
ILMN_2634564 230.1194 32 18.81391 0
ILMN_2737647 26.40867 43 5.434363 0.04010025
ILMN_2734484 341.1319 41 27.36952 0
ILMN_2952292 77.78281 41 7.908901 0
ILMN_2699078 4.936621 39 3.344758 0.6516291
ILMN_1213681 597.907 52 30.54501 0
ILMN_2735413 10.01557 35 5.628383 0.4047619
ILMN_2735415 31.6327 32 6.746305 0.02130326
ILMN_2891688 688.7415 41 45.90011 0
ILMN_2637698 2171.392 32 116.2498 0
ILMN_2674228 444.3493 43 27.37509 0
ILMN_2601546 16.13324 29 6.372973 0.1879699
ILMN_1230831 14.32738 33 5.312377 0.2406015
ILMN_2848071 31.24027 29 7.520035 0.02506266

Total number of rows: 25697

Table truncated, full table size 1008 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap