|
| Status |
Public on Feb 08, 2012 |
| Title |
Spinal cord, acrylamide treated, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Rat pups treated with acrylamide, spinal cord
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
gender: male
tissue: spinal cord
treatment: acrylamide
|
| Treatment protocol |
25-day-old rats were treated with either solvent or acrylamide daily (30 mg/kg) via oral gavage. Tissues (cerebellum, right and left sciatic nerves, and spinal cord) were collected 21 days after treatment.
|
| Growth protocol |
25-day-old male Wistar rats were housed two per cage and maintained in a 12 h light / 12 h dark cycle with food (Purina 5001) and water provided ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The Qiagen RNeasy Lipid Tissue Midi-kit was used for total RNA extraction.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Hybridization protocol, and GeneChip Fluidics Station 450 default protocol were used. GeneChip Rat Genome 230 2.0 arrays were used.
|
| Scan protocol |
Affymetrix GenChip Scanner 3000 default parameters were used.
|
| Description |
ACRL_SC__Treated_B
Gene expression data from acrylamide-treated rat pups.
|
| Data processing |
Image analysis, scaling (TGT = 500), and probeset-level data analysis was performed using default parameters in GCOS v1.1.
|
| |
|
| Submission date |
May 02, 2011 |
| Last update date |
Feb 08, 2012 |
| Contact name |
Ameeta Agarwal |
| E-mail(s) |
aagarwal@olemiss.edu
|
| Phone |
662-915-1218
|
| Organization name |
University of Mississippi
|
| Department |
National Center for Natural Products Research
|
| Street address |
NCNPR, Room 2049
|
| City |
University |
| State/province |
MS |
| ZIP/Postal code |
38677 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE29004 |
Gene expression response to acrylamide in rat pups |
|
