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Sample GSM71927 Query DataSets for GSM71927
Status Public on Aug 26, 2005
Title 4 Hr. Timepoint
Sample type RNA
 
Source name whole cell
Organism Saccharomyces cerevisiae
Characteristics Tetracycline regulated-Paf1 (TetO2-Paf1) strain grown in the presence of doxycycline for 4 hrs.
Extracted molecule total RNA
Extraction protocol Whole cell extracts were prepared from liquid cultures of TetO2-Paf1 grown to mid-log phase in the presence of doxycyclinefor 4 hrs.
Label biotin
Label protocol Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description TetO2-Paf1 strain grown in the presence of doxycycline for 4 hrs.
Data processing Affymetrix Microarray Suite version 4.0
 
Submission date Aug 25, 2005
Last update date Aug 25, 2005
Contact name Judith A. Jaehning
E-mail(s) Judith.Jaehning@uchsc.edu
Organization name UCHSC
Department Biochemistry and Molecular Genetics
Street address 4200 E. 9th Avenue Campus Box B-121
City Denver
State/province CO
ZIP/Postal code 80262
Country USA
 
Platform ID GPL90
Series (1)
GSE3200 Post-Transcriptional Role for the Yeast Paf1-RNA Polymerase II Complex is Revealed by Identification of Primary Targets

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 3.8 A 0.979978
AFFX-MurIL10_at 4.4 A 0.659339
AFFX-MurIL4_at 0.8 A 0.9273
AFFX-MurFAS_at 1.9 A 0.672921
AFFX-BioB-5_at 386.5 P 0.00039
AFFX-BioB-M_at 524.1 P 0.000052
AFFX-BioB-3_at 534.8 P 0.000081
AFFX-BioC-5_at 1168.7 P 0.000052
AFFX-BioC-3_at 1260.8 P 0.000044
AFFX-BioDn-5_at 1840.3 P 0.000044
AFFX-BioDn-3_at 10379.1 P 0.000044
AFFX-CreX-5_at 8878.3 P 0.000044
AFFX-CreX-3_at 10250.2 P 0.000044
AFFX-BioB-5_st 17.4 A 0.617401
AFFX-BioB-M_st 22.6 A 0.51489
AFFX-BioB-3_st 6.2 A 0.772364
AFFX-BioC-5_st 10.6 A 0.699394
AFFX-BioC-3_st 4.6 A 0.617401
AFFX-BioDn-5_st 30.6 A 0.51489
AFFX-BioDn-3_st 14.1 A 0.275146

Total number of rows: 9335

Table truncated, full table size 225 Kbytes.




Supplementary data files not provided

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