|
Status |
Public on Aug 26, 2005 |
Title |
4 Hr. Timepoint |
Sample type |
RNA |
|
|
Source name |
whole cell
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
Tetracycline regulated-Paf1 (TetO2-Paf1) strain grown in the presence of doxycycline for 4 hrs.
|
Extracted molecule |
total RNA |
Extraction protocol |
Whole cell extracts were prepared from liquid cultures of TetO2-Paf1 grown to mid-log phase in the presence of doxycyclinefor 4 hrs.
|
Label |
biotin
|
Label protocol |
Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
|
|
|
Hybridization protocol |
standard Affymetrix procedures
|
Scan protocol |
standard Affymetrix procedures
|
Description |
TetO2-Paf1 strain grown in the presence of doxycycline for 4 hrs.
|
Data processing |
Affymetrix Microarray Suite version 4.0
|
|
|
Submission date |
Aug 25, 2005 |
Last update date |
Aug 25, 2005 |
Contact name |
Judith A. Jaehning |
E-mail(s) |
Judith.Jaehning@uchsc.edu
|
Organization name |
UCHSC
|
Department |
Biochemistry and Molecular Genetics
|
Street address |
4200 E. 9th Avenue Campus Box B-121
|
City |
Denver |
State/province |
CO |
ZIP/Postal code |
80262 |
Country |
USA |
|
|
Platform ID |
GPL90 |
Series (1) |
GSE3200 |
Post-Transcriptional Role for the Yeast Paf1-RNA Polymerase II Complex is Revealed by Identification of Primary Targets |
|