|
| Status |
Public on Aug 26, 2005 |
| Title |
8 Hr. Timepoint |
| Sample type |
RNA |
| |
|
| Source name |
whole cell
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Tetracycline regulated-Paf1 (TetO2-Paf1) strain grown in the presence of doxycycline for 8 hrs.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Whole cell extracts were prepared from liquid cultures of TetO2-Paf1 grown to mid-log phase in the presence of doxycyclinefor 8 hrs.
|
| Label |
biotin
|
| Label protocol |
Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
TetO2-Paf1 strain grown in the presence of doxycycline for 8 hrs.
|
| Data processing |
Affymetrix Microarray Suite version 4.0
|
| |
|
| Submission date |
Aug 25, 2005 |
| Last update date |
Aug 25, 2005 |
| Contact name |
Judith A. Jaehning |
| E-mail(s) |
Judith.Jaehning@uchsc.edu
|
| Organization name |
UCHSC
|
| Department |
Biochemistry and Molecular Genetics
|
| Street address |
4200 E. 9th Avenue Campus Box B-121
|
| City |
Denver |
| State/province |
CO |
| ZIP/Postal code |
80262 |
| Country |
USA |
| |
|
| Platform ID |
GPL90 |
| Series (1) |
| GSE3200 |
Post-Transcriptional Role for the Yeast Paf1-RNA Polymerase II Complex is Revealed by Identification of Primary Targets |
|
