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Sample GSM71992 Query DataSets for GSM71992
Status Public on Aug 26, 2006
Title FACS-purified B cells_wildtype_non-infected_rep1
Sample type RNA
 
Source name B cells - wildtype mice - not infected
Organism Mus musculus
Characteristics Strain: 129SV/EV
Gender: Female
Age: 8-12weeks
Tissue: Lymph node B cell
Noninfected

Biomaterial provider B&K Universal Ltd.- U. K.
Treatment protocol None
Growth protocol Mice were bred and held under conventional housing conditions in filtertop cages
Extracted molecule total RNA
Extraction protocol RNAeasy kit (Qiagen)
Label Biotin
Label protocol Bioarray (Enzo)
 
Hybridization protocol Hybridized washed and stained with streptavidin-PE per the manufacturer's protocol (Affymetrix).
Scan protocol Affymetrix GeneChip Scanner 3000 and GCOS software using target value = 250
Description B cells from control mice (uninfected wild type)
Array 15 - 15.N.B._467.CEL
Data processing The dataset was preprocessed using the following procedures: (1) background correction; (2) normalization using the quantile method (Bolstad et al. 2003); (3) summarization of probe set values using the RMA (Robust Multi-array Analysis) method, which fits a specified robust linear model to the probe level data. The abovementioned procedures were performed using the Bioconductor package “affyPLM”.
 
Submission date Aug 25, 2005
Last update date Aug 28, 2018
Contact name Michael E. Salazar
Phone (415) 514-4371
URL http://arrays.ucsf.edu
Organization name University of California, San Francisco
Department
Lab Functional Genomics Core Laboratories
Street address 1550 Fourth Street, RM 545
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL1261
Series (1)
GSE3203 Influenza virus infection-induced gene expression changes of regional B cells are mediated in part through type I IFN
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA normalized intensity

Data table
ID_REF VALUE
1415670_at 8.675
1415671_at 10.852
1415672_at 11.32
1415673_at 8.525
1415674_a_at 9.523
1415675_at 9.388
1415676_a_at 11.453
1415677_at 8.473
1415678_at 10.334
1415679_at 11.58
1415680_at 10.015
1415681_at 9.415
1415682_at 8.891
1415683_at 10.583
1415684_at 8.282
1415685_at 7.997
1415686_at 9.959
1415687_a_at 12.596
1415688_at 10.686
1415689_s_at 9.536

Total number of rows: 45101

Table truncated, full table size 764 Kbytes.




Supplementary file Size Download File type/resource
GSM71992.CEL.gz 6.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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