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Sample GSM72002 Query DataSets for GSM72002
Status Public on Aug 26, 2006
Title FACS-purified B cells_IFNAR1-/-_influenza-infected_rep3
Sample type RNA
Source name B cells - IFNAR1-/-mice - influenza-infected
Organism Mus musculus
Characteristics Strain: 129SV/EV IFNAR1-/-
Gender: Female
Age: 8-12weeks
Tissue: Mediastinal lymph node B cell
Influenza A/Mem71 48h

Biomaterial provider B&K Universal Ltd.- U. K.
Treatment protocol Infected with influenza A/Mem71 virus for 2 days
Growth protocol Mice were bred and held under conventional housing conditions in filtertop cages
Extracted molecule total RNA
Extraction protocol RNAeasy kit (Qiagen)
Label Biotin
Label protocol Bioarray (Enzo)
Hybridization protocol Hybridized washed and stained with streptavidin-PE per the manufacturer's protocol (Affymetrix).
Scan protocol Affymetrix GeneChip Scanner 3000 and GCOS software using target value = 260
Description B cells from flu-infected mice (IFNR knockout)
Array 27 - 27.N.B._620.CEL
Data processing The dataset was preprocessed using the following procedures: (1) background correction; (2) normalization using the quantile method (Bolstad et al. 2003); (3) summarization of probe set values using the RMA (Robust Multi-array Analysis) method, which fits a specified robust linear model to the probe level data. The abovementioned procedures were performed using the Bioconductor package “affyPLM”.
Submission date Aug 25, 2005
Last update date Aug 28, 2018
Contact name Michael E. Salazar
Phone (415) 514-4371
Organization name University of California, San Francisco
Lab Functional Genomics Core Laboratories
Street address 1550 Fourth Street, RM 545
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platform ID GPL1261
Series (1)
GSE3203 Influenza virus infection-induced gene expression changes of regional B cells are mediated in part through type I IFN
Reanalyzed by GSE119085

Data table header descriptions
VALUE RMA normalized intensity

Data table
1415670_at 8.87
1415671_at 9.876
1415672_at 11.515
1415673_at 7.345
1415674_a_at 8.114
1415675_at 8.533
1415676_a_at 10.299
1415677_at 7.313
1415678_at 9.927
1415679_at 9.808
1415680_at 10.071
1415681_at 8.74
1415682_at 7.973
1415683_at 9.861
1415684_at 7.646
1415685_at 8.776
1415686_at 10.519
1415687_a_at 11.378
1415688_at 10.669
1415689_s_at 10.473

Total number of rows: 45101

Table truncated, full table size 764 Kbytes.

Supplementary file Size Download File type/resource
GSM72002.CEL.gz 6.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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