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Sample GSM72003 Query DataSets for GSM72003
Status Public on Aug 26, 2006
Title FACS-purified B cells_IFNAR1-/-_influenza-infected_rep4
Sample type RNA
Source name B cells - IFNAR1-/-mice - influenza-infected
Organism Mus musculus
Characteristics Strain: 129SV/EV IFNAR1-/-
Gender: Female
Age: 8-12weeks
Tissue: Mediastinal lymph node B cell
Influenza A/Mem71 48h

Biomaterial provider B&K Universal Ltd.- U. K.
Treatment protocol Infected with influenza A/Mem71 virus for 2 days
Growth protocol Mice were bred and held under conventional housing conditions in filtertop cages
Extracted molecule total RNA
Extraction protocol RNAeasy kit (Qiagen)
Label Biotin
Label protocol Bioarray (Enzo)
Hybridization protocol Hybridized washed and stained with streptavidin-PE per the manufacturer's protocol (Affymetrix).
Scan protocol Affymetrix GeneChip Scanner 3000 and GCOS software using target value = 261
Description B cells from flu-infected mice (IFNR knockout)
Array 32 - 32.N.B._630.CEL
Data processing The dataset was preprocessed using the following procedures: (1) background correction; (2) normalization using the quantile method (Bolstad et al. 2003); (3) summarization of probe set values using the RMA (Robust Multi-array Analysis) method, which fits a specified robust linear model to the probe level data. The abovementioned procedures were performed using the Bioconductor package “affyPLM”.
Submission date Aug 25, 2005
Last update date Aug 28, 2018
Contact name Michael E. Salazar
Phone (415) 514-4371
Organization name University of California, San Francisco
Lab Functional Genomics Core Laboratories
Street address 1550 Fourth Street, RM 545
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platform ID GPL1261
Series (1)
GSE3203 Influenza virus infection-induced gene expression changes of regional B cells are mediated in part through type I IFN
Reanalyzed by GSE119085

Data table header descriptions
VALUE RMA normalized intensity

Data table
1415670_at 9.02
1415671_at 10.253
1415672_at 11.295
1415673_at 7.872
1415674_a_at 9.037
1415675_at 8.99
1415676_a_at 11.416
1415677_at 7.656
1415678_at 10.103
1415679_at 11.054
1415680_at 9.851
1415681_at 9.665
1415682_at 8.309
1415683_at 10.552
1415684_at 7.963
1415685_at 8.475
1415686_at 10.269
1415687_a_at 11.633
1415688_at 10.468
1415689_s_at 9.741

Total number of rows: 45101

Table truncated, full table size 764 Kbytes.

Supplementary file Size Download File type/resource
GSM72003.CEL.gz 6.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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