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Sample GSM72004 Query DataSets for GSM72004
Status Public on Aug 26, 2006
Title FACS-purified B cells_129SV/EV_IFN-b-stimulated_rep1
Sample type RNA
Source name B cells - wildtype mice - IFN-b stimulated
Organism Mus musculus
Characteristics Strain: 129SV/EV
Gender: Female
Age: 8-12weeks
Tissue: Lymph node B cell
17h treatment IFN-beta (2000U/ml)

Biomaterial provider B&K Universal Ltd.- U. K.
Treatment protocol In vitro exposure to IFN-b (17 hours, 2000U/ml)
Growth protocol Mice were bred and held under conventional housing conditions in filtertop cages
Extracted molecule total RNA
Extraction protocol RNAeasy kit (Qiagen)
Label Biotin
Label protocol Bioarray (Enzo)
Hybridization protocol Hybridized washed and stained with streptavidin-PE per the manufacturer's protocol (Affymetrix).
Scan protocol Affymetrix GeneChip Scanner 3000 and GCOS software using target value = 262
Description B cells from wild type mice treated with IFN-b
Array 23 - 23.N.B._565.CEL
Data processing The dataset was preprocessed using the following procedures: (1) background correction; (2) normalization using the quantile method (Bolstad et al. 2003); (3) summarization of probe set values using the RMA (Robust Multi-array Analysis) method, which fits a specified robust linear model to the probe level data. The abovementioned procedures were performed using the Bioconductor package “affyPLM”.
Submission date Aug 25, 2005
Last update date Aug 28, 2018
Contact name Michael E. Salazar
Phone (415) 514-4371
Organization name University of California, San Francisco
Lab Functional Genomics Core Laboratories
Street address 1550 Fourth Street, RM 545
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platform ID GPL1261
Series (1)
GSE3203 Influenza virus infection-induced gene expression changes of regional B cells are mediated in part through type I IFN
Reanalyzed by GSE119085

Data table header descriptions
VALUE RMA normalized intensity

Data table
1415670_at 8.723
1415671_at 9.623
1415672_at 11.678
1415673_at 7.271
1415674_a_at 8.381
1415675_at 8.345
1415676_a_at 10.595
1415677_at 7.208
1415678_at 9.915
1415679_at 9.974
1415680_at 9.948
1415681_at 9.144
1415682_at 7.949
1415683_at 10.536
1415684_at 7.558
1415685_at 8.229
1415686_at 9.893
1415687_a_at 10.717
1415688_at 10.883
1415689_s_at 9.825

Total number of rows: 45101

Table truncated, full table size 764 Kbytes.

Supplementary file Size Download File type/resource
GSM72004.CEL.gz 6.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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