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Sample GSM72007 Query DataSets for GSM72007
Status Public on Aug 26, 2006
Title FACS-purified B cells_129SV/EV_IFN-b-stimulated_rep4
Sample type RNA
 
Source name B cells - wildtype mice - IFN-b stimulated
Organism Mus musculus
Characteristics Strain: 129SV/EV
Gender: Female
Age: 8-12weeks
Tissue: Lymph node B cell
17h treatment IFN-beta (2000U/ml)

Biomaterial provider B&K Universal Ltd.- U. K.
Treatment protocol In vitro exposure to IFN-b (17 hours, 2000U/ml)
Growth protocol Mice were bred and held under conventional housing conditions in filtertop cages
Extracted molecule total RNA
Extraction protocol RNAeasy kit (Qiagen)
Label Biotin
Label protocol Bioarray (Enzo)
 
Hybridization protocol Hybridized washed and stained with streptavidin-PE per the manufacturer's protocol (Affymetrix).
Scan protocol Affymetrix GeneChip Scanner 3000 and GCOS software using target value = 265
Description B cells from wild type mice treated with IFN-b
Array 34 - 34.N.B._638.CEL
Data processing The dataset was preprocessed using the following procedures: (1) background correction; (2) normalization using the quantile method (Bolstad et al. 2003); (3) summarization of probe set values using the RMA (Robust Multi-array Analysis) method, which fits a specified robust linear model to the probe level data. The abovementioned procedures were performed using the Bioconductor package “affyPLM”.
 
Submission date Aug 25, 2005
Last update date Aug 28, 2018
Contact name Michael E. Salazar
Phone (415) 514-4371
URL http://arrays.ucsf.edu
Organization name University of California, San Francisco
Department
Lab Functional Genomics Core Laboratories
Street address 1550 Fourth Street, RM 545
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL1261
Series (1)
GSE3203 Influenza virus infection-induced gene expression changes of regional B cells are mediated in part through type I IFN
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA normalized intensity

Data table
ID_REF VALUE
1415670_at 8.934
1415671_at 10.17
1415672_at 10.895
1415673_at 7.969
1415674_a_at 8.904
1415675_at 9.11
1415676_a_at 11.716
1415677_at 6.936
1415678_at 9.885
1415679_at 11.002
1415680_at 10.12
1415681_at 10.09
1415682_at 7.929
1415683_at 11.371
1415684_at 7.375
1415685_at 8.275
1415686_at 9.664
1415687_a_at 11.091
1415688_at 10.091
1415689_s_at 8.908

Total number of rows: 45101

Table truncated, full table size 764 Kbytes.




Supplementary file Size Download File type/resource
GSM72007.CEL.gz 6.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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